LOCUS       Exported                1040 bp ds-DNA     linear   SYN 27-MAY-2021
DEFINITION  Expresses OmpR constitutively from PompB97 and sfGFP under the 
            PompF146 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pSR59.4
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Schmidl SR, Sheth RU, Wu A, Tabor JJ
  TITLE     Refactoring and optimization of light-switchable escherichia coli 
            two-component systems.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. 
            Epub 2014 Oct 11.
  PUBMED    25250630
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 27, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      460..531
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      547..574
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tgtgcaggaa cgcacgattt cctttaagga tgacggcacg tacaaaacgc gtgcggaagt
       61 gaaatttgaa ggcgataccc tggtaaaccg cattgagctg aaaggcattg actttaaaga
      121 agacggcaat atcctgggcc ataagctgga atacaatttt aacagccaca atgtttacat
      181 caccgccgat aaacaaaaaa atggcattaa agcgaatttt aaaattcgcc acaacgtgga
      241 ggatggcagc gtgcagctgg ctgatcacta ccagcaaaac actccaatcg gtgatggtcc
      301 tgttctgctg ccagacaatc actatctgag cacgcaaagc gttctgtcta aagatccgaa
      361 cgagaaacgc gatcatatgg ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg
      421 tatggatgaa ctgtacaaat gatgaagatc tccaggcatc aaataaaacg aaaggctcag
      481 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
      541 cacactggct caccttcggg tgggcctttc tgcgtttata gtaaatcact gcataattcg
      601 tgtcgattaa accgcctaag tcccccagga aagggggata taacagtata gattttgtca
      661 gccttcagct tggctttacc gtcaaaaaaa taggctgaaa ttcataccag atttagctgg
      721 tgacgaacgt gagctttttt aagaatacac gcttacaaat tgttgcgaac ctttgggagt
      781 acaaacaatg caagagaact acaagattct ggtggtcgat gacgacatgc gcctgcgtgc
      841 gctgctggaa cgttatctca ccgaacaagg cttccaggtt cgaagcgtcg ctaatgcaga
      901 acagatggat cgcctgctga ctcgtgaatc tttccatctt atggtactgg atttaatgtt
      961 acctggtgaa gatggcttgt cgatttgccg acgtcttcgt agtcagagca acccgatgcc
     1021 gatcattatg gtgacggcga
//
