LOCUS       Exported                 757 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    HA Raptor
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Kim DH, Sarbassov DD, Ali SM, King JE, Latek RR, Erdjument-Bromage 
            H, Tempst P, Sabatini DM
  TITLE     mTOR interacts with raptor to form a nutrient-sensitive complex that
            signals to the cell growth machinery.
  JOURNAL   Cell 2002 Jul 26;110(2):163-75.
  PUBMED    12150925
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      211..220
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    224..358
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(261..280)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     315..334
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(450..470)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     697..716
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 caatcacgac gttaaccggc tcgagagagc gccggcctag aggctcatta tctatttatt
       61 ttaccaaacg cgaattgaga cggactttga caaaacacga aatggtaatg tgaagctaag
      121 agcagagagt gaccaacagt aaacaacacg cgcagactca aaaaaaaaaa aaaaagggcg
      181 gccgctaagt aagtaaggat ccccagcttg gccgccatgg cccaacttgt ttattgcagc
      241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
      301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcgg
      361 gaattaattc ggcgcagcac catggcctga aataacctct gaaagaggaa cttggttagg
      421 taccttctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
      481 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
      541 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
      601 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      661 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
      721 ctcggcctct gagctattcc agaagtagtg aggaggc
//
