LOCUS Exported 811 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Protein expression.. ACCESSION . VERSION . KEYWORDS pCri-8a SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 811) AUTHORS Goulas T, Cuppari A, Garcia-Castellanos R, Snipas S, Glockshuber R, Arolas JL, Gomis-Ruth FX TITLE The pCri System: A Vector Collection for Recombinant Protein Expression and Purification. JOURNAL PLoS One. 2014 Nov 11;9(11):e112643. doi: 10.1371/journal.pone.0112643. eCollection 2014. PUBMED 25386923 REFERENCE 2 (bases 1 to 811) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..811 /organism="synthetic DNA construct" /mol_type="other DNA" RBS 2..24 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 43..60 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 70..90 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS 103..618 /codon_start=1 /product="N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VN173 /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIE" primer_bind complement(145..166) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(406..425) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 753..774 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 ttttgtttaa ctttaagaag gagatatcat atgggcagca gccatcatca tcatcatcac 61 agcagcggcg aaaaccttta cttccagggc gccatggcca aagtgagcaa gggcgaggag 121 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag 181 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc 241 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac 301 ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc 361 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac 421 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag 481 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac 541 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag 601 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc 661 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc 721 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc 781 gccgggatca ctctcggcat ggacgagctg t //