LOCUS       Exported                7018 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Ubiquitin promoter driving synthetic optimised GUSPlus gene. Can be 
            used as a reporter of promoter activity in plants by replacing Ubi 
            promoter with sequence of interest..
ACCESSION   .
VERSION     .
KEYWORDS    pUbiGUSPlus
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Vickers CE, Xue GP, Gresshoff PM
  TITLE     A synthetic xylanase as a novel reporter in plants.
  JOURNAL   Plant Cell Rep. 2003 Sep;22(2):135-40. Epub 2003 Jul 4.
  PUBMED    12845475
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     91..110
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     304..326
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     318..335
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     319..335
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        351..2342
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             join(2389..2403,2594..4411)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          2404..2593
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             4418..4435
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4467..4719
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4737..4753)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4737..4753)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4750..4772)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4761..4777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4785..4815)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4830..4851
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4968..4985)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5139..5727)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5219..5238)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5898..6758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6521..6540
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6759..6863)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6931..6949
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6987..7009)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
       61 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      121 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      181 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      241 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      301 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcagtgca
      361 gcgtgacccg gtcgtgcccc tctctagaga taatgagcat tgcatgtcta agttataaaa
      421 aattaccaca tatttttttt gtcacacttg tttgaagtgc agtttatcta tctttataca
      481 tatatttaaa ctttactcta cgaataatat aatctatagt actacaataa tatcagtgtt
      541 ttagagaatc atataaatga acagttagac atggtctaaa ggacaattga gtattttgac
      601 aacaggactc tacagtttta tctttttagt gtgcatgtgt tctccttttt ttttgcaaat
      661 agcttcacct atataatact tcatccattt tattagtaca tccatttagg gtttagggtt
      721 aatggttttt atagactaat ttttttagta catctatttt attctatttt agcctctaaa
      781 ttaagaaaac taaaactcta ttttagtttt tttatttaat aatttagata taaaatagaa
      841 taaaataaag tgactaaaaa ttaaacaaat accctttaag aaattaaaaa aactaaggaa
      901 acatttttct tgtttcgagt agataatgcc agcctgttaa acgccgtcga cgagtctaac
      961 ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca
     1021 tctctgtcgc tgcctctgga cccctctcga gagttccgct ccaccgttgg acttgctccg
     1081 ctgtcggcat ccagaaattg cgtggcggag cggcagacgt gagccggcac ggcaggcggc
     1141 ctcctcctcc tctcacggca cggcagctac gggggattcc tttcccaccg ctccttcgct
     1201 ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt tccccaacct
     1261 cgtgttgttc ggagcgcaca cacacacaac cagatctccc ccaaatccac ccgtcggcac
     1321 ctccgcttca aggtacgccg ctcgtcctcc cccccccccc ctctctacct tctctagatc
     1381 ggcgttccgg tccatggtta gggcccggta gttctacttc tgttcatgtt tgtgttagat
     1441 ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag
     1501 acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg atggctctag
     1561 ccgttccgca gacgggatcg atttcatgat tttttttgtt tcgttgcata gggtttggtt
     1621 tgcccttttc ctttatttca atatatgccg tgcacttgtt tgtcgggtca tcttttcatg
     1681 cttttttttg tcttggttgt gatgatgtgg tctggttggg cggtcgttct agatcggagt
     1741 agaattctgt ttcaaactac ctggtggatt tattaatttt ggatctgtat gtgtgtgcca
     1801 tacatattca tagttacgaa ttgaagatga tggatggaaa tatcgatcta ggataggtat
     1861 acatgttgat gcgggtttta ctgatgcata tacagagatg ctttttgttc gcttggttgt
     1921 gatgatgtgg tgtggttggg cggtcgttca ttcgttctag atcggagtag aatactgttt
     1981 caaactacct ggtgtattta ttaattttgg aactgtatgt gtgtgtcata catcttcata
     2041 gttacgagtt taagatggat ggaaatatcg atctaggata ggtatacatg ttgatgtggg
     2101 ttttactgat gcatatacat gatggcatat gcagcatcta ttcatatgct ctaaccttga
     2161 gtacctatct attataataa acaagtatgt tttataatta ttttgatctt gatatacttg
     2221 gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc atacgctatt
     2281 tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg ttacttctgc
     2341 aggtcgactc tagaggatcc gtcctaaagc aagcaagagc aataaacaat ggtagatctg
     2401 agggtaaatt tctagttttt ctccttcatt ttcttggtta ggaccctttt ctctttttat
     2461 ttttttgagc tttgatcttt ctttaaactg atctattttt taattgattg gttatggtgt
     2521 aaatattaca tagctttaac tgataatctg attactttat ttcgtgtgtc tatgatgatg
     2581 atgatagtta cagaaccgac gaactagtct gtacccgatc aacaccgaga cccgtggcgt
     2641 cttcgacctc aatggcgtct ggaacttcaa gctggactac gggaaaggac tggaagagaa
     2701 gtggtacgaa agcaagctga ccgacactat tagtatggcc gtcccaagca gttacaatga
     2761 cattggcgtg accaaggaaa tccgcaacca tatcggatat gtctggtacg aacgtgagtt
     2821 cacggtgccg gcctatctga aggatcagcg tatcgtgctc cgcttcggct ctgcaactca
     2881 caaagcaatt gtctatgtca atggtgagct ggtcgtggag cacaagggcg gattcctgcc
     2941 attcgaagcg gaaatcaaca actcgctgcg tgatggcatg aatcgcgtca ccgtcgccgt
     3001 ggacaacatc ctcgacgata gcaccctccc ggtggggctg tacagcgagc gccacgaaga
     3061 gggcctcgga aaagtcattc gtaacaagcc gaacttcgac ttcttcaact atgcaggcct
     3121 gcaccgtccg gtgaaaatct acacgacccc gtttacgtac gtcgaggaca tctcggttgt
     3181 gaccgacttc aatggcccaa ccgggactgt gacctatacg gtggactttc aaggcaaagc
     3241 cgagaccgtg aaagtgtcgg tcgtggatga ggaaggcaaa gtggtcgcaa gcaccgaggg
     3301 cctgagcggt aacgtggaga ttccgaatgt catcctctgg gaaccactga acacgtatct
     3361 ctaccagatc aaagtggaac tggtgaacga cggactgacc atcgatgtct atgaagagcc
     3421 gttcggcgtg cggaccgtgg aagtcaacga cggcaagttc ctcatcaaca acaaaccgtt
     3481 ctacttcaag ggctttggca aacatgagga cactcctatc aacggccgtg gctttaacga
     3541 agcgagcaat gtgatggatt tcaatatcct caaatggatc ggcgccaaca gcttccggac
     3601 cgcacactat ccgtactctg aagagttgat gcgtcttgcg gatcgcgagg gtctggtcgt
     3661 gatcgacgag actccggcag ttggcgtgca cctcaacttc atggccacca cgggactcgg
     3721 cgaaggcagc gagcgcgtca gtacctggga gaagattcgg acgtttgagc accatcaaga
     3781 cgttctccgt gaactggtgt ctcgtgacaa gaaccatcca agcgtcgtga tgtggagcat
     3841 cgccaacgag gcggcgactg aggaagaggg cgcgtacgag tacttcaagc cgttggtgga
     3901 gctgaccaag gaactcgacc cacagaagcg tccggtcacg atcgtgctgt ttgtgatggc
     3961 taccccggag acggacaaag tcgccgaact gattgacgtc atcgcgctca atcgctataa
     4021 cggatggtac ttcgatggcg gtgatctcga agcggccaaa gtccatctcc gccaggaatt
     4081 tcacgcgtgg aacaagcgtt gcccaggaaa gccgatcatg atcactgagt acggcgcaga
     4141 caccgttgcg ggctttcacg acattgatcc agtgatgttc accgaggaat atcaagtcga
     4201 gtactaccag gcgaaccacg tcgtgttcga tgagtttgag aacttcgtgg gtgagcaagc
     4261 gtggaacttc gcggacttcg cgacctctca gggcgtgatg cgcgtccaag gaaacaagaa
     4321 gggcgtgttc actcgtgacc gcaagccgaa gctcgccgcg cacgtctttc gcgagcgctg
     4381 gaccaacatt ccagatttcg gctacaagaa cgctagccat caccatcacc atcacgtgtg
     4441 aattggtgac cagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa
     4501 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     4561 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     4621 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     4681 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattcgta atcatggtca
     4741 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4801 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4861 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     4921 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4981 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5041 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5101 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5161 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5221 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5281 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5341 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5401 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5461 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5521 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     5581 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5641 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5701 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5761 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5821 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5881 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5941 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6001 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6061 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6121 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6181 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6241 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6301 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6361 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6421 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6481 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6541 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6601 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6661 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6721 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6781 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6841 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     6901 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     6961 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtca
//
