LOCUS Exported 743 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS. ACCESSION . VERSION . KEYWORDS pME-Cas9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 743) AUTHORS Ablain J, Durand EM, Yang S, Zhou Y, Zon LI TITLE A CRISPR/Cas9 Vector System for Tissue-Specific Gene Disruption in Zebrafish. JOURNAL Dev Cell. 2015 Mar 4. pii: S1534-5807(15)00075-1. doi: 10.1016/j.devcel.2015.01.032. PUBMED 25752963 REFERENCE 2 (bases 1 to 743) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..743 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(32..52) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" RBS 147..155 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" ORIGIN 1 gcggtggcgg ccgctctaga ttccgcggtc acacctttct cttcttctta ggagatctca 61 ccggtgagtc gccacccagc tgagacaggt caatccttgt ttcgtacagg ccggtaatac 121 tctgatggat cagtgtagcg tccagcacct ccttagtgga ggtgtacctc tttctatcga 181 ttgtagtgtc gaagtacttg aaagctgcag gtgcgcccag gtttgtcaga gtgaacagat 241 gaatgatatt ttcggcctgc tccctgatgg gcttatctct gtgtttgttg taagcagaca 301 ggaccttatc cagatttgcg tcggccagga tcactctttt agagaactcg ctaatctgtt 361 caatgatctc gtccagataa tgcttgtgct gctccacaaa cagctgtttc tgttcgttat 421 cctctggaga tcctttcagc ttctcgtagt ggcttgccag atacaggaag ttcacgtact 481 tagaaggcag ggccagttca ttgcctttct gcagctctcc agcgcttgcc agcatgcgtt 541 tcctgccgtt ttccagctca aacagactgt acttgggcag tttaatgatc aggtctttct 601 tcacttcctt ataacctttg gcctccagaa aatcgattgg gttcttctcg aaagagcttc 661 tctccataat tgtgataccc agcagctcct tgacagattt cagtttcttg cttttgccct 721 tctccacctt agcgaccacc agg //