LOCUS Exported 859 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION This plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.. ACCESSION . VERSION . KEYWORDS pMarsL274G SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 859) AUTHORS Mahdavi A, Hamblin GD, Jindal GA, Bagert JD, Dong C, Sweredoski MJ, Hess S, Schuman EM, Tirrell DA TITLE Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of Mammalian Protein Synthesis. JOURNAL J Am Chem Soc. 2016 Apr 6;138(13):4278-81. doi: 10.1021/jacs.5b08980. Epub 2016 Mar 25. PUBMED 26991063 REFERENCE 2 (bases 1 to 859) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..859 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 34..53 /label=T7 /note="T7 promoter, forward primer" promoter 34..52 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 72..81 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 84..101 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" ORIGIN 1 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca 61 agctggctag cgccaccatg gcccaccatc accatcacca tatgagactg ttcgtgagcg 121 agggttcccc ggggagcctg cccgtgctgg ctgcggccgc gagggcccgg ggtcgggcgg 181 agctgctcat cagcaccgta ggccccgaag agtgtgtggt accattcctt acccggccta 241 aggtccctgt cttgcagctg gatagtggca actacctctt ctctgctagt gcaatctgcc 301 gatatttttt tctgttatgt ggctgggaac aagatgatct caccaaccag tggctggaat 361 gggaggcaac agaactgcag ccagttctgt ctgctgccct acactgtcta gtggttcaag 421 gcaagaaagg ggaagatata cttggcccac ttcggagagt cctgactcac attgatcaca 481 gcttgagtcg tcaaaactgt cctttcctgg ctggggacac agaatctcta gctgacattg 541 ttttgtgggg agcactgtat cctttactgc aagacccagc ttacctccct gaggagttgg 601 gtgccctgca aagttggttc cagacactga gtacccagga accgtgtcag cgagctgcag 661 agacggtgct aaaacagcag ggtgtcctgg cacttcgtct gtacctccag aaacagccac 721 agcctcagcc cccgcctcct gaggggagaa ctgtcagcaa cgagctggag gaagaggaac 781 tggctacctt gtctgaggag gacatcgtta cagctgttgc cgcgtgggag aagggtctgg 841 aaagctgctc cctaactca //