LOCUS       Exported                 892 bp ds-DNA     linear   SYN 25-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    LacZ in pLX304
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Rosenbluh J, Nijhawan D, Cox AG, Li X, Neal JT, Schafer EJ, Zack TI,
            Wang X, Tsherniak A, Schinzel AC, Shao DD, Schumacher SE, Weir BA, 
            Vazquez F, Cowley GS, Root DE, Mesirov JP, Beroukhim R, Kuo CJ, 
            Goessling W, Hahn WC
  TITLE     beta-Catenin-Driven Cancers Require a YAP1 Transcriptional Complex 
            for Survival and Tumorigenesis.
  JOURNAL   Cell. 2012 Dec 21;151(7):1457-73. doi: 10.1016/j.cell.2012.11.026. 
            Epub 2012 Dec 13.
  PUBMED    23245941
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 25, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     389..411
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    complement(428..452)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(475..678)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(504..524)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acgccatcaa aaataattcg cgtctggcct tcctgtagcc agctttcatc aacattaaat
       61 gtgagcgagt aacaacccgt cggattctcc gtgggaacaa acggcggatt gaccgtaatg
      121 ggataggtca cgttggtgta gatgggcgca tcgtaaccgt gcatctgcca gtttgagggg
      181 acgacgacag tatcggcctc aggaagatcg cactccagcc agctttccgg caccgcttct
      241 ggtgccggaa accaggcaaa gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg
      301 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
      361 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacgggatct
      421 atcatggtgc caactttttt gtacaaactt gttgatcccg acagttagcc agagagctct
      481 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
      541 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
      601 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
      661 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
      721 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
      781 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
      841 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg tt
//
