LOCUS       Exported                1515 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    FLAG-YAP2 (8SA)-pcw107
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggactaca aagacgatga cgataaagca aggctcgaat cggtacctaa ggatcccggg
       61 cagcagccgc cgcctcaacc ggccccccag ggccaagggc agccgccttc gcagcccccg
      121 caggggcagg gcccgccgtc cggacccggg caaccggcac ccgcggcgac ccaggcggcg
      181 ccgcaggcac cccccgccgg gcatcagatc gtgcacgtcc gcggggacgc ggagaccgac
      241 ctggaggcgc tcttcaacgc cgtcatgaac cccaagacgg ccaacgtgcc ccagaccgtg
      301 cccatgaggc tccggaagct gcccgactcc ttcttcaagc cgccggagcc caaatcccac
      361 tcccgacagg ccgctactga tgcaggcact gcaggagccc tgactccaca gcatgttcga
      421 gctcatgccg ctccagctgc tctgcagttg ggagctgttt ctcctgggac actgaccccc
      481 actggagtag tctctggccc agcagctaca cccacagctc agcatcttcg acaggctgct
      541 tttgagatac ctgatgatgt acctctgcca gcaggttggg agatggcaaa gacatcttct
      601 ggtcagagat acttcttaaa tcacatcgat cagacaacaa catggcagga ccccaggaag
      661 gccatgctgt cccagatgaa cgtcacagcc cccaccagtc caccagtgca gcagaatatg
      721 atgaactcgg cttcaggtcc tcttcctgat ggatgggaac aagccatgac tcaggatgga
      781 gaaatttact atataaacca taagaacaag accacctctt ggctagaccc aaggcttgac
      841 cctcgttttg ccatgaacca gagaatcagt cagagtgctc cagtgaaaca gccaccaccc
      901 ctggctcccc agagcccaca gggaggcgtc atgggtggca gcaactccaa ccagcagcaa
      961 cagatgcgac tgcagcaact gcagatggag aaggagaggc tgcggctgaa acagcaagaa
     1021 ctgcttcggc aggagttagc cctgtgtagc cagttaccaa cactggagca ggatggtggg
     1081 actcaaaatc cagtgtcttc tcccgggatg tctcaggaat tgagaacaat gacgaccaat
     1141 agctcagatc ctttccttaa cagtggcacc tatcactctc gagatgaggc tacagacagt
     1201 ggactaagca tgagcagcta cagtgtccct cgaaccccag atgacttcct gaacagtgtg
     1261 gatgagatgg atacaggtga tactatcaac caaagcaccc tgccctcaca gcagaaccgt
     1321 ttcccagact accttgaagc cattcctggg acaaatgtgg accttggaac actggaagga
     1381 gatggaatga acatagaagg agaggagctg atgccaagtc tgcaggaagc tttgagttct
     1441 gacatcctta atgacatgga gtctgttttg gctgccacca agctagataa agaaagcttt
     1501 cttacatggt tatag
//
