LOCUS       Exported                 497 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT 
            core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA-dCas9-p300 Core (D1399Y)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy
            TE, Gersbach CA
  TITLE     Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates
            genes from promoters and enhancers.
  JOURNAL   Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 
            2015 Apr 6.
  PUBMED    25849900
REFERENCE   2  (bases 1 to 497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 cgcaaagttg ggcgcgccat tttcaaacca gaagaactac gacaggcact gatgccaact
       61 ttggaggcac tttaccgtca ggatccagaa tcccttccct ttcgtcaacc tgtggaccct
      121 cagcttttag gaatccctga ttactttgat attgtgaaga gccccatgga tctttctacc
      181 attaagagga agttagacac tggacagtat caggagccct ggcagtatgt cgatgatatt
      241 tggcttatgt tcaataatgc ctggttatat aaccggaaaa catcacgggt atacaaatac
      301 tgctccaagc tctctgaggt ctttgaacaa gaaattgacc cagtgatgca aagccttgga
      361 tactgttgtg gcagaaagtt ggagttctct ccacagacac tgtgttgcta cggcaaacag
      421 ttgtgcacaa tacctcgtga tgccacttat tacagttacc agaacaggta tcatttctgt
      481 gagaagtgtt tcaatga
//
