LOCUS       Exported                 722 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  Expresses MCP-VP64 and dCas9 in Yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    pJZC638
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(137..157)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(161..181)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(185..205)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tagagtatat acaactgtat aagatagtaa taaaaatatc gcactcacgt aaacacttaa
       61 tcataatatt aattcgtata taatttagct atttgcttag agctccaccg cggtggcggc
      121 cgccggtaga attctatacc tttctcttct tttttggatc tacctttctc ttcttttttg
      181 gatctacctt tctcttcttt tttggatcag ctccctcatc ccctccgagc tgtgagaggt
      241 caatccgagt ttcgtaaagt ccggtgatgc tctggtggat gagggtggcg tccagcactt
      301 ctttggtgga ggtatagcgt ttccggtcga tggtggtgtc gaagtacttg aatgcggcag
      361 gggcaccaag attggtgagg gtgaacaggt ggatgatatt ctcagcctgc tcccgaattg
      421 gtttgtctct gtgcttattg tatgcgctaa ggactttatc gaggttggca tcagcgagaa
      481 tgacgcgttt agaaaactcg gaaatctgct caatgatctc gtccagataa tgcttatgct
      541 gctccacaaa cagttgcttt tgctcgttgt cctcggggct ccccttcagc ttctcgtaat
      601 ggcttgcaag gtagaggaaa ttcacgtact tggaggggag agccagctca ttacccttct
      661 gaagttcgcc agcggaggcg agcatccgct tccgcccatt ctccagttcg aacaggctat
      721 ac
//
