LOCUS Exported 895 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.. ACCESSION . VERSION . KEYWORDS lentiGuide-Puro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 895) AUTHORS Sanjana NE, Shalem O, Zhang F TITLE Improved vectors and genome-wide libraries for CRISPR screening. JOURNAL Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. PUBMED 25075903 REFERENCE 2 (bases 1 to 895) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..895 /organism="synthetic DNA construct" /mol_type="other DNA" promoter complement(527..738) /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" ORIGIN 1 ccgcccaggg cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag 61 cttgagacta cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac 121 atgtgcgctg ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag 181 tgtgcagatc ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag 241 gtcatcaaaa attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt 301 gccaccagat tcgcacgcgg cggccccagc gcccaggcca ggcctcaact caagcacgag 361 gcgaaggggc tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct 421 cgggatcaag aatcacgtac tgcagccagg tggaagtaat tcaaggcacg caagggccat 481 aacccgtaaa gaggccaggc ccgcgggaac cacacacggc acttacctgt gttctggcgg 541 caaacccgtt gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc 601 caccctcggg aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc 661 accttctcta ggcaccggat caattgccga cccctccccc caacttctcg gggactgtgg 721 gcgatgtgcg ctctgcccac tgacgggcac cggagccaat tcccactcct ttcaagacct 781 agaaggtcca ttagctgcaa agattcctct ctgtttaaaa ctttatccat ctttgcaccc 841 gggccccctc gagccggcgc caaagtggat ctctgctgtc cctgtaataa acccg //