LOCUS Exported 9676 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.. ACCESSION . VERSION . KEYWORDS TetO-FUW-NfiB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9676) AUTHORS Caiazzo M, Giannelli S, Valente P, Lignani G, Carissimo A, Sessa A, Colasante G, Bartolomeo R, Massimino L, Ferroni S, Settembre C, Benfenati F, Broccoli V TITLE Direct conversion of fibroblasts into functional astrocytes by defined transcription factors. JOURNAL Stem Cell Reports. 2015 Jan 13;4(1):25-36. doi: 10.1016/j.stemcr.2014.12.002. Epub 2014 Dec 31. PUBMED 25556566 REFERENCE 2 (bases 1 to 9676) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9676 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 7..386 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 388..586 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 541..561 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 604..784 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 831..956 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1449..1682 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1867..1911 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2209..2326 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 2383..2653 /label=tetracycline response element /bound_moiety="tetracycline repressor TetR" /note="contains seven copies of the tetracycline operator tetO" promoter 2686..2723 /label=minimal CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2720..2744 /label=LNCX /note="Human CMV promoter, forward primer" misc_feature 4137..4725 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4190..4210) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(4608..4619) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(4728..4744) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4729..4745) /label=pBluescriptKS /note="For pBluescript vector" LTR 5250..5430 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(5456..5473) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5462..5686 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 5732..6160 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(5819..5838) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6029..6050 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6169..6189) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6174..6503 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6354..6489 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6416..6435 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 6551..6598 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 6617..6991 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 7121..7242 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7158..7177) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7212..7231 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7291..7307) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7291..7307) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7304..7326) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7315..7331 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7339..7369) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7384..7405 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7522..7539) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7693..8281) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7773..7792) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8452..9312) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9075..9094 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9313..9417) /gene="bla" /label=AmpR promoter primer_bind complement(9492..9511) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt 601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga 1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga 2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg 2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa 2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag 2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag 2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt 2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg 2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt 2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta 2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgagtagg cgtgtacggt 2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc 2761 cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc ggccccgaat 2821 tcgttaacgg atccatgatg tattctccca tctgtctcac tcaggatgaa ttccacccat 2881 ttattgaggc acttcttcct cacgtccgtg caattgccta tacttggttc aacctgcagg 2941 ctcgaaaacg caagtacttt aaaaagcatg agaaacgaat gtcgaaggat gaagaaaggg 3001 cagtcaaaga cgagctgctc agtgagaagc ccgaaatcaa gcagaagtgg gcatccaggc 3061 tcctggccaa actgcgcaaa gatatccgcc aggagtaccg ggaggacttt gtgcttaccg 3121 tgactggcaa gaagcacccg tgctgtgtct tatccaatcc agaccagaag ggtaagatta 3181 ggaggatcga ctgcctgcga caggcagaca aagtctggcg tctggatcta gtcatggtga 3241 tcctgttcaa aggcatccct ttggagagta cggatggaga gcgactcatg aagtccccgc 3301 actgcacaaa cccagcactt tgtgttcagc cacaccacat cacagtatca gttaaggagc 3361 ttgacttgtt tttggcatac tacgtgcagg agcaagattc tggacaatca ggaagtccaa 3421 gccacagtga tcctgccaag aatcctccag ggtacctcga ggacagcttt gtaaaatccg 3481 gagtcttcaa tgtatcagag cttgtgagag tatccagaac acccataacc cagggaactg 3541 gagtcaactt cccaatcgga gaaattccca gccaaccata ctatcatgac atgaactctg 3601 gtgtgaacct gcagaggtcg ctgtcttctc caccgagcag caaaagaccc aaaactatat 3661 ctatagatga aaatatggag ccaagtccta caggagactt ttacccctct ccaaattcac 3721 cagctgctgg aagtcgaaca tggcatgaac gagatcaaga tatgtcttct ccaactacaa 3781 tgaagaagcc tgagaagcca ctgtttagct ctacatctcc acaggattct tccccaagat 3841 tgagcacttt cccccagcac catcatcccg gaatacctgg agtcgcgcac agtgtcatct 3901 caactcgaac tccacctccg ccctcaccgt tgccatttcc gacgcaagct atccttcctc 3961 cggcaccttc cagctacttc tctcatccaa caatcagata tcctcctcac ctgaatcctc 4021 aggatactct gaagaactac gtaccttctt atgacccatc cagtcctcaa acgagccagt 4081 cctggtacct gggctagtct agataaagcg ctcaattcga tatcaagctt atcgataatc 4141 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 4201 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 4261 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 4321 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 4381 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 4441 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 4501 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct 4561 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 4621 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 4681 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac 4741 ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg 4801 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg 4861 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa 4921 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga 4981 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca 5041 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag 5101 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg 5161 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca 5221 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct 5281 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 5341 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 5401 ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg 5461 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc 5521 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 5581 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 5641 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 5701 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg 5761 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 5821 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 5881 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 5941 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 6001 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 6061 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 6121 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc 6181 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 6241 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 6301 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 6361 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 6421 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt 6481 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg 6541 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa 6601 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga 6661 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga 6721 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga 6781 ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta 6841 cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac 6901 cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg 6961 cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc 7021 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 7081 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 7141 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 7201 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 7261 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 7321 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 7381 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 7441 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 7501 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 7561 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 7621 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 7681 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 7741 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 7801 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 7861 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 7921 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 7981 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 8041 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 8101 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 8161 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 8221 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 8281 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 8341 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 8401 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 8461 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 8521 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 8581 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 8641 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 8701 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 8761 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 8821 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 8881 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 8941 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 9001 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 9061 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 9121 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 9181 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 9241 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 9301 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 9361 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 9421 atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg 9481 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct 9541 tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc 9601 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat 9661 gtacgggcca gatata //