LOCUS Exported 12799 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pFThGW SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12799) AUTHORS Dittgen T, Nimmerjahn A, Komai S, Licznerski P, Waters J, Margrie TW, Helmchen F, Denk W, Brecht M, Osten P TITLE Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo. JOURNAL Proc Natl Acad Sci U S A. 2004 Dec 28. 101(52):18206-11. PUBMED 15608064 REFERENCE 2 (bases 1 to 12799) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12799 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" regulatory 6725..6734 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 6731..7450 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 6731..7447 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6776..6797) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(7037..7056) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 7384..7405 /label=EGFP-C /note="EGFP, forward primer" misc_feature 7491..8079 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(7544..7564) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(7962..7973) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(8082..8098) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8083..8099) /label=pBluescriptKS /note="For pBluescript vector" LTR 8604..8784 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(8810..8827) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8816..9040 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 9086..9514 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9173..9192) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9383..9404 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(9523..9543) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 9528..9857 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 9708..9843 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9770..9789 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 9905..9952 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 9971..10345 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 10475..10596 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(10512..10531) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 10566..10585 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(10645..10661) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10645..10661) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10658..10680) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10669..10685 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10693..10723) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10738..10759 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(10876..10893) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(11047..11635) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(11127..11146) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(11806..12666) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 12429..12448 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(12667..12771) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaaatt cagagaccgg gaaccaaact agcctttaaa aaacataagt 2641 acaggagcca gcaagatggc tcagtgggta aaggtgccta ccagcaagcc tgacagcctg 2701 agttcagtcc ccacgaacta cgtggtagga gaggaccaac caactctgga aatctgttct 2761 gcaaacacat gctcacacac acacacacaa atagtataaa caattttaaa tttcatttaa 2821 aaataatttg taaacaaaat cattagcaca ggttttagaa agagcctctt ggtgacatca 2881 agttgatgct gtagatgggg tatcattcct gaggacccaa aaccgggtct cagcctttcc 2941 ccattctgag agttctctct tttctcagcc actagctgaa gagtagagtg gctcagcact 3001 gggctcttga gttcccaagt cctacaactg gtcagcctga ctactaacca gccatgaaga 3061 aacaaggagt ggatgggctg agtctgctgg gatgggagtg gagttagtaa gtggccatgg 3121 atgtaatgac cccagcaatg ctggctagaa ggcatgcctc ctttccttgt ctggagacgg 3181 aacgggaggg atcatcttgt actcacagaa gggagaacat tctagctggt tgggccaaaa 3241 tgtgcaagtt cacctggagg tggtggtgca tgcttttaac tccagtactc aggaggcagg 3301 gccaggtgga tctctgtgag ttcaagacca gcctgcacta tggagagagt tttgggacag 3361 ccagagttac acagaaaaat cctggtggaa aatctgaaag aaagagagaa agaaagaaag 3421 aaagaaagga agaaagaaag aaagagtggc aggcaggcag gcaggaggaa ggaaggaagg 3481 aaggaaggaa ggaaggaagg aaggaaggaa aataggtgcg acttcaagat ccggagttac 3541 aagcagaatg cactgtttcc ctaacagggc caagtgtttt gagtaactga aggtgggcat 3601 gatgcctagg aagcgaaaca agccaagcag atgcacccct tgcctttctt ccgaagggct 3661 gcagtagcat ggaaaagatg gaaaacaacc aatccattcc ctttgctgat ataacaggct 3721 ccaaagccaa aacctgtcac tggaggctca agagcagatc tccagccaag aggcaaagga 3781 atgggggaag ctggagggcc tccctctggt tatccaggct tctgaaggtt caagcaaaga 3841 aagggttaca accttaaaag gagagcgtcc cggggtatgg gtagaagact gctccacccc 3901 gacccccagg gtccctaacc gtcttttcct tgggcgagtc agcccaatca caggactgag 3961 agtgcctctt tagtagcagc aagccacttc ggacacccaa atggaacacc tccagtcagc 4021 cctcgccgac caccccaccc cctccatcct tttccctcag cctccgattg gctgaatcta 4081 gagtccctcc ctgctccccc ctctctcccc acccctggtg aaaactgcgg gcttcagcgc 4141 tgggtgcagc aactggaggc gttggcgcac caggaggagg ctgcagctag gggagtccag 4201 gtgagagcag gccgacggga gggacccgca catgcaagga ccgccgcagg gcgaggatgc 4261 aagccttccc cagctacagt tttgggaaag gataccaggg cgctcctata tgggggcgcg 4321 ggaactgggg aaagaaggtg ctcccaggtc gaggtgggag aggaaggcag tgcggggtca 4381 cgggctttct ccctgctaac ggacgctttc gaagagtggg tgccggagga gaaccatgag 4441 gaaggacatc aaggacagcc tttggtcccc aagctcaaat cgctttagtg gtgcgaatag 4501 agggaggagg tgggtggcaa actggaggga gtccccagcg ggtgacctcg tggctggctg 4561 ggtgcggggc accgcaggta agaaaaccgc aatgttgcgg gaggggactg ggtggcaggc 4621 gcgggggagg ggaaagctag aaaggatgcg agggagcgga ggggggaggg agcgggagaa 4681 tctcaactgg tagaggaaga ttaaaatgag gaaatagcat cagggtgggg ttagccaagc 4741 cgggcctcag ggaaagggcg caaagtttgt ctgggtgtgg gcttaggtgg gctgggtatg 4801 agattcgggg cgccgaaaac actgctgcgc ctctgccaaa tcacgctacc cctgtatcta 4861 gttctgccag gcttctccag ccccagcccc aattcttttc tctagtgtcc ccttccctcc 4921 cctgaatctc aagcccacac tccctcctcc ataacccact gttatcaaat ctaagtcatt 4981 tgccacccaa caaccatcag gaggcggaag cagacgggag gagtttgaga tcaacttggg 5041 ctacatcacg agttccaggc tcaccaaggc ttcttaagga gaccttgtct ctaaaattaa 5101 ttaattaatt aattaatagt cccctttctc tgccacagaa ccttgggatc tggctcctgg 5161 tcgcagctcc ccccacccca ggctgacatt cactgccata gcccatccgg aaatcctagt 5221 ctatttcccc atggatcttg aactgcagag agaatggcag agtggcccgc cctgtgcaaa 5281 ggatgttcct agcctaggtg gagctcgcga actcgcagac tgtgcctctc ttgggcaagg 5341 acaggctaga cagcctgccg gtgtgttgag ctagggcact gtggggaagg cagagaacct 5401 gtgcagggca gcaatgaaca caggaccaga aaactgcagc cctaggaaca ctcaagagct 5461 ggccatttgc aagcatctct ggcctccgtg cttctcactc atgtcccatg tcttatacag 5521 gcctctgtgg cacctcgctt gcctgatctc atccctagcc gttaagcttt ctgcatgact 5581 tatcacttgg ggcataatgc tggataccta ccattttctt agaccccatc aaaatcctat 5641 ttgagtgtac ggttcggaga acctcattta tccggtaaat gtcttttact ctgctctcag 5701 ggagctgagg caggacatcc tgagatacat tgggagagga gatacagttt caataaaata 5761 ataggttggg tggaggtaca tgcctataat gccaccactc aggaaatggt ggcagcttcg 5821 tgagtttgag gccaacccaa gaaacatagt gaaaccctgt cagtaaataa gtaagcaagt 5881 atttgagtat ctactatatg ctagggctga cctggacatt aggggtcatc ttctgaacaa 5941 actagtgctt gagggaggta tttggggttt ttgtttgttt aatggatctg aatgagttcc 6001 agagactggc tacacagcga tatgactgag cttaacaccc ctaaagcata cagtcagacc 6061 aattagacaa taaaaggtat gtatagctta ccaaataaaa aaattgtatt ttcaagagag 6121 tgtctgtctg tgtagccctg gctgttcttg aactcactct gtagaccagg ctggcctgga 6181 aatccatctg cctgcctctg cctctctgcc tctctgcctc tctgcctctc tctctgcctc 6241 tctctgcctc tctctgcccc tctctgcccc tctctgcccc tctctgccgc cctctgcctt 6301 ttgccctctg ccctctgttc tctggcctct gccctctgcc ctctggcctc tggcctctgc 6361 ctctgcctct tgagtgctgg aatcaaaggt gtgagctctg taggtcttaa gttccagaag 6421 aaagtaatga agtcacccag cagggaggtg ctcagggaca gcacagacac acacccagga 6481 cataggctcc cacttccttg gctttctctg agtggcaaag gaccttaggc agtgtcactc 6541 cctaagagaa ggggataaag agaggggctg aggtattcat catgtgctcc gtggatctca 6601 agccctcaag gtaaatgggg acccacctgt cctaccagct ggctgacctg tagctttccc 6661 caccacagaa tccaagtcgg aactcttggc acctagagga tctcgagatc cccgggtacc 6721 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 6781 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 6841 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 6901 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga 6961 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 7021 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 7081 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 7141 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 7201 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt 7261 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 7321 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 7381 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 7441 gtacaagtaa agcggccgcg actctagaat tcgatatcaa gcttatcgat aatcaacctc 7501 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 7561 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 7621 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 7681 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 7741 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 7801 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 7861 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg 7921 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 7981 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 8041 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag 8101 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg 8161 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt 8221 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 8281 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc 8341 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc 8401 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag 8461 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc 8521 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc 8581 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc 8641 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc 8701 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt 8761 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg 8821 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 8881 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 8941 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 9001 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 9061 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 9121 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 9181 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 9241 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 9301 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 9361 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 9421 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 9481 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 9541 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 9601 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 9661 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 9721 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 9781 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 9841 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc 9901 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag 9961 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc 10021 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga 10081 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt 10141 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga 10201 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat 10261 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca 10321 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt 10381 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 10441 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg 10501 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 10561 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc 10621 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 10681 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 10741 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 10801 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 10861 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 10921 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 10981 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 11041 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 11101 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 11161 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 11221 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 11281 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 11341 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 11401 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 11461 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 11521 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 11581 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 11641 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 11701 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 11761 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 11821 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 11881 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 11941 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 12001 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 12061 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 12121 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 12181 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 12241 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 12301 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 12361 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 12421 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 12481 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 12541 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 12601 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 12661 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 12721 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 12781 ccgaaaagtg ccacctgac //