LOCUS       Exported                 786 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  shRNA against human pro-caspase 1.
ACCESSION   .
VERSION     .
KEYWORDS    shRNA Caspase 1 (pSicoR mCherry)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Doitsh G, Galloway NL, Geng X, Yang Z, Monroe KM, Zepeda O, Hunt PW,
            Hatano H, Sowinski S, Munoz-Arias I, Greene WC
  TITLE     Cell death by pyroptosis drives CD4 T-cell depletion in HIV-1 
            infection.
  JOURNAL   Nature. 2014 Jan 23;505(7484):509-14. doi: 10.1038/nature12940.
  PUBMED    24356306
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    complement(77..110)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(408..427)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     677..695
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttttgtatcc agaggttgat tatcgataag cttgatatcg aattgtacct agtggaaccg
       61 gaacccttaa acatgtataa cttcgtataa tgtatgctat acgaagttat taggtccctc
      121 gacgaattct tacttgtaca gctcgtccat gccgccggtg gagtggcggc cctcggcgcg
      181 ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca acttgatgtt
      241 gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg tcttgacctc
      301 agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc ccttcagggc
      361 gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggtct tcttctgcat
      421 tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc
      481 gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac
      541 gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga tgtcggcggg
      601 gtgcttcacg taggccttgg agccgtacat gaactgaggg gacaggatgt cccaggcgaa
      661 gggcaggggg ccacccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
      721 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac
      781 cttgaa
//
