LOCUS       Exported                 903 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    Cre-IRES-PuroR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Somers A, Jean JC, Sommer CA, Omari A, Ford CC, Mills JA, Ying L, 
            Sommer AG, Jean JM, Smith BW, Lafyatis R, Demierre MF, Weiss DJ, 
            French DL, Gadue P, Murphy GJ, Mostoslavsky G, Kotton DN
  TITLE     Generation of transgene-free lung disease-specific human induced 
            pluripotent stem cells using a single excisable lentiviral stem cell
            cassette.
  JOURNAL   Stem Cells. 2010 Oct . 28(10):1728-40.
  PUBMED    20715179
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(53..652)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(136..156)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     633..652
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(826..845)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 agtcaatctt tcacaaattt tgtaatccag aggttgatta ggatctatcg atttaggcac
       61 cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga
      121 cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg
      181 cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac
      241 ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg
      301 gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca
      361 actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg
      421 tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc
      481 cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccggat
      541 cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc
      601 tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atatgtgtgg
      661 ccatattatc atcgtgtttt tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga
      721 cgttttttta acctcgacta aacacatgta aagcatgtgc accgaggccc cagatcagat
      781 cccatacaat ggggtacctt ctgggcatcc ttcagcccct tgttgaatac gcttgaggag
      841 agccatttga ctctttccac aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc
      901 ttt
//
