LOCUS       Exported                 810 bp ds-DNA     linear   SYN 17-AUG-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pMH4-Syn1-Ratio1XsypHy
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Rose T, Schoenenberger P, Jezek K, Oertner TG
  TITLE     Developmental refinement of vesicle cycling at schaffer collateral 
            synapses.
  JOURNAL   Neuron. 2013 Mar 20;77(6):1109-21. doi: 
            10.1016/j.neuron.2013.01.021.
  PUBMED    23522046
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    99..233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(123..142)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     177..196
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(309..332)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(507..526)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     724..744
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     776..794
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ggcggcggct gctgcaaaac agatacaaaa ctacataaga cccccacctt atatattctt
       61 tcccaccctt aaccacgccc agatcttcga tgctagacga tccagacatg ataagataca
      121 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
      181 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttgctcgaa
      241 gtcgacctac aggaacaggt ggtggcggcc ctcggagcgc tcgtactgtt ccacgatggt
      301 gtagtcctcg ttgtgggagg tgatgtccag cttggtgtcc acgtagtagt agccgggcag
      361 ctgcacgggc ttcttggcca tgtagatggt cttgaactcc accaggtagt ggccgccgtc
      421 cttcagcttc agggcctggt ggatctcgcc cttcagcacg ccgtcgcggg ggtacaggcg
      481 ctcggtggag gcctcccagc ccatggtctt cttctgcatt acggggccgt cgggggggaa
      541 gttggtgccg cggaacttca ccttgtagat cagcgtgccg tcctgcaggg aggagtcctg
      601 ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc
      661 ggggaaggac agcttcttgt agtcggggat gtcggcgggg tgcttcacgt acgccttgga
      721 gccgtactgg aactgggggg acaggatgtc ccaggcgaag ggcagggggc cgcccttggt
      781 caccttcagc ttggcggtct gggtgccctc
//
