LOCUS       Exported                 855 bp ds-DNA     linear   SYN 03-SEP-2021
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal tDimer2 Strep-tag II followed by HIS5 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    pGS1613
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 3, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(183..201)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(233..253)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     451..470
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     645..668
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ttcgtacgct gcaggtcgac ggatcggtga cggtgctggt ttaattaaca tggtggcctc
       61 ctccgaggac gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa
      121 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      181 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
      241 ccagttccag tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgactacaa
      301 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      361 cgtggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa
      421 gttccgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg
      481 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca
      541 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc
      601 caagaagccc gtgcagctgc ccggctacta ctacgtggac accaagctgg acatcacctc
      661 ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct
      721 gttcctgggg catggcaccg gcagcaccgg cagcggcagc tccggcaccg cctcctccga
      781 ggacgtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca
      841 cgagttcgag atcga
//
