LOCUS Exported 1856 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Encodes ConLS as a Type 1 part to be used in the Dueber YTK system. ACCESSION . VERSION . KEYWORDS pYTK002 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1856) AUTHORS Lee ME, DeLoache WC, Cervantes B, Dueber JE TITLE A Highly-characterized Yeast Toolkit for Modular, Multi-part Assembly. JOURNAL ACS Synth Biol. 2015 Apr 14. PUBMED 25871405 REFERENCE 2 (bases 1 to 1856) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1856 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 221..255 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(326..985) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 900..919 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1167..1755 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1656..1675 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tcggtctcac cctgaattcg catctagatg gtagagccac aaacagccgg tacaagcaac 61 gatctccagg accatctgaa tcatgcgcgg atgacacgaa ctcacgacgg cgatcacaga 121 cattaaccca cagtacagac actgcgacaa cgtggcaatt cgtcgcaata ccgtctcact 181 gaactggccg ataattgcag acgaacgtga gaccagacca ataaaaaacg cccggcggca 241 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag 301 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt 361 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa 421 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg 481 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg 541 gattggctga aacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt 601 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt 661 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt 721 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag 781 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct 841 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag 901 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg 961 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact 1021 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt 1081 gcccgatcaa tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1801 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtag //