LOCUS Exported 2371 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Encodes pTEF1 as a Type 2 part to be used in the Dueber YTK system. ACCESSION . VERSION . KEYWORDS pYTK013 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2371) AUTHORS Lee ME, DeLoache WC, Cervantes B, Dueber JE TITLE A Highly-characterized Yeast Toolkit for Modular, Multi-part Assembly. JOURNAL ACS Synth Biol. 2015 Apr 14. PUBMED 25871405 REFERENCE 2 (bases 1 to 2371) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2371 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 293..700 /gene="S. cerevisiae TEF1" /label=TEF1 promoter /note="promoter for EF-1-alpha" terminator 736..770 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(841..1500) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1415..1434 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1682..2270 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2171..2190 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tcggtctcaa acgccttgcc aacagggagt tcttcagaga catggaggct caaaacgaaa 61 ttattgacag cctagacatc aatagtcata caacagaaag cgaccaccca actttggctg 121 ataatagcgt ataaacaatg catactttgt acgttcaaaa tacaatgcag tagatatatt 181 tatgcatatt acatataata catatcacat aggaagcaac aggcgcgttg gacttttaat 241 tttcgaggac cgcgaatcct tacatcacac ccaatccccc acaagtgatc ccccacacac 301 catagcttca aaatgtttct actccttttt tactcttcca gattttctcg gactccgcgc 361 atcgccgtac cacttcaaaa cacccaagca cagcatacta aatttcccct ctttcttcct 421 ctagggtgtc gttaattacc cgtactaaag gtttggaaaa gaaaaaagac accgcctcgt 481 ttctttttct tcgtcgaaaa aggcaataaa aatttttatc acgtttcttt ttcttgaaaa 541 tttttttttt tgattttttt ctctttcgat gacctcccat tgatatttaa gttaataaac 601 ggtcatcaat ttctcaagtt tcagtttcat ttttcttgtt ctattacaac tttttttact 661 tcttgctcat tagaaagaaa gcatagcaat ctaatctaag ttttaattac aaaagatcta 721 tgtgagacca gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga 781 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa 841 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac 901 atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc 961 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc 1021 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt 1081 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga 1141 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt 1201 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc 1261 accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg 1321 aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat 1381 atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg 1441 ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat 1501 tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct 1561 tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc 1621 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 1681 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 1741 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 1801 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 1861 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 1921 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 1981 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 2041 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 2101 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 2161 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 2221 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 2281 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 2341 cgttatcccc tgattctgtg gataaccgta g //