LOCUS Exported 2370 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Encodes pHTB2 as a Type 2 part to be used in the Dueber YTK system. ACCESSION . VERSION . KEYWORDS pYTK016 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2370) AUTHORS Lee ME, DeLoache WC, Cervantes B, Dueber JE TITLE A Highly-characterized Yeast Toolkit for Modular, Multi-part Assembly. JOURNAL ACS Synth Biol. 2015 Apr 14. PUBMED 25871405 REFERENCE 2 (bases 1 to 2370) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2370 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 735..769 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(840..1499) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1414..1433 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1681..2269 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2170..2189 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tcggtctcaa acgtatatat taaatttgct cttgttctgt actttcctaa ttcttatgta 61 aaaagacaag aatttatgat actatttaat aacaaaaaac tacctaagaa aagcatcatg 121 cagtcgaaat tgaaatcgaa aagtaaaact ttaacggaac atgtttgaaa ttctaagaaa 181 gcatacatct tcatccctta tatatagagt tatgtttgat attagtagtc atgttgtaat 241 ctctggccta agtatacgta acgaaaatgg tagcacgtcg cgtttatggc ccccaggtta 301 atgtgttctc tgaaattcgc atcactttga gaaataatgg gaacacctta cgcgtgagct 361 gtgcccaccg cttcgcctaa taaagcggtg ttctcaaaat ttctccccgt tttcaggatc 421 acgagcgcca tctagttctg gtaaaatcgc gcttacaaga acaaagaaaa gaaacatcgc 481 gtaatgcaac agtgagacac ttgccgtcat atataaggtt ttggatcagt aaccgttatt 541 tgagcataac acaggttttt aaatatatta ttatatatca tggtatatgt gtaaaatttt 601 tttgctgact ggttttgttt atttatttag ctttttaaaa attttacttt cttcttgtta 661 attttttctg attgctctat actcaaacca acaacaactt actctacaac taagatctat 721 gtgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat 781 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat 841 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca 901 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg 961 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc 1021 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc 1081 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa 1141 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt 1201 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca 1261 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga 1321 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata 1381 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt 1441 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt 1501 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt 1561 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc 1621 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 1681 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 1741 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 1801 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 1861 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 1921 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 1981 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 2041 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 2101 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 2161 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 2221 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 2281 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 2341 gttatcccct gattctgtgg ataaccgtag //