LOCUS Exported 1835 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Encodes ConR4 as a Type 5 part to be used in the Dueber YTK system. ACCESSION . VERSION . KEYWORDS pYTK070 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1835) AUTHORS Lee ME, DeLoache WC, Cervantes B, Dueber JE TITLE A Highly-characterized Yeast Toolkit for Modular, Multi-part Assembly. JOURNAL ACS Synth Biol. 2015 Apr 14. PUBMED 25871405 REFERENCE 2 (bases 1 to 1835) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1835 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 200..234 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(305..964) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 879..898 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1146..1734 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1635..1654 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tcggtctcag ctgggtatga gacggcctgc actagacgaa ctaggcaaga tgcgtccaat 61 ccgtctaaac atggtgacaa cgctggacag atgacgtaac accgagccac atcctgaaat 121 cgaggcaggc taaccgaaac cgtgacaatg caaagagaca gcctgacact agtgcactgc 181 agtacatgag accagaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc 241 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg agctcgatat 301 caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc 361 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct 421 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat 481 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgaa acgaaaaaca 541 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt 601 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa 661 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca 721 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa 781 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg 841 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa 901 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct 961 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg 1021 atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaat catgaccaaa 1081 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 1141 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 1201 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 1261 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 1321 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 1381 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 1441 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 1501 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 1561 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 1621 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 1681 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 1741 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 1801 cctgcgttat cccctgattc tgtggataac cgtag //