LOCUS Exported 2174 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Encodes CEN6/ARS4 as a Type 7 part to be used in the Dueber YTK system. ACCESSION . VERSION . KEYWORDS pYTK081 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2174) AUTHORS Lee ME, DeLoache WC, Cervantes B, Dueber JE TITLE A Highly-characterized Yeast Toolkit for Modular, Multi-part Assembly. JOURNAL ACS Synth Biol. 2015 Apr 14. PUBMED 25871405 REFERENCE 2 (bases 1 to 2174) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2174 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 14..517 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence" terminator 539..573 /label=lambda t0 terminator /note="minimal transcription terminator from phage lambda (Scholtissek and Grosse, 1987)" CDS complement(644..1303) /codon_start=1 /gene="cat" /product="chloramphenicol acetyltransferase" /label=CmR /note="confers resistance to chloramphenicol" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" primer_bind 1218..1237 /label=CAT-R /note="Chloramphenicol resistance gene, reverse primer" rep_origin 1485..2073 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1974..1993 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tcggtctcag agtatcacgt gctataaaaa taattataat ttaaattttt taatataaat 61 atataaatta aaaatagaaa gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc 121 gaagatgtaa aagactctag ggggatcgcc aacaaatact accttttatc ttgctcttcc 181 tgctctcagg tattaatgcc gaattgtttc atcttgtctg tgtagaaaac cacacacgaa 241 aatcctgtga ttttacattt tacttatcgt taatcgaatg tatatctatt taatctgctt 301 ttcttgtcta ataaatatat atgtaaagta cgctttttgt tgaaattttt taaacctttg 361 tttatttttt tttcttcatt ccgtaactct tctaccttct ttatttactt tctaaaatcc 421 aaatacaaaa cataaaaata aataaacaca gagtaaattc ccaaattatt ccatcattaa 481 aagatacgag gcgcgtgtaa gttacaggca agcgatccgt cccgatgaga ccagaccaat 541 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat 601 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc 661 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa 721 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt 781 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac 841 tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag 901 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact 961 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga 1021 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca 1081 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa 1141 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct 1201 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt 1261 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc 1321 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa 1381 agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg 1441 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 1501 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 1561 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 1621 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 1681 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 1741 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 1801 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 1861 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 1921 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 1981 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 2041 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 2101 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 2161 gtggataacc gtag //