LOCUS       Exported                2906 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Encodes AmpR-ColE1 as a Type 678 part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    pYTK095
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly-characterized Yeast Toolkit for Modular, Multi-part 
            Assembly.
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(116..976)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     739..758
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(977..1081)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1184..1772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1673..1692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     RBS             2041..2052
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2059..2772
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      2784..2855
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2871..2898
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tcggtctcat acagcggccg cgattatcaa aaaggatctt cacctagatc cttttaaatt
       61 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
      121 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
      181 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
      241 ctgcaatgat accgcgggac ccacgctcac cggctccaga tttatcagca ataaaccagc
      301 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
      361 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
      421 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
      481 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
      541 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
      601 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
      661 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
      721 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
      781 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
      841 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
      901 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
      961 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1021 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     1081 gcacatttcc ccgaaaagtg ccacctgtca tgaccaaaat cccttaacgt gagttttcgt
     1141 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1201 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1261 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1321 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1381 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1441 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1501 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1561 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1621 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1681 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1741 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1801 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1861 tggataaccg tgcggccgcc ccttgagacc atgaaagtga aacgtgattt catgcgtcat
     1921 tttgaacatt ttgtaaatct tatttaataa tgtgtgcggc aattcacatt taatttatga
     1981 atgttttctt aacatcgcgg caactcaaga aacggcaggt tcggatctta gctactagag
     2041 aaagaggaga aatactagat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt
     2101 ctggtggaac tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa
     2161 ggtgacgcaa ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg
     2221 gttccttggc cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat
     2281 ccggaccata tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag
     2341 gaacgcacga tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt
     2401 gaaggcgata ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaggacggc
     2461 aatatcctgg gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc
     2521 gataaacaaa aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc
     2581 agcgtgcagc tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg
     2641 ctgccagaca atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa
     2701 cgcgatcata tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat
     2761 gaactgtaca aatgaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2821 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2881 cgggtgggcc tttctgcgtt tataag
//
