LOCUS Exported 8994 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION TNF reporter only (no hook) (RUSH system). ACCESSION . VERSION . KEYWORDS pCDH_TNF-SBP-EGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8994) AUTHORS Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche L, Mercanti V, Jollivet F, Raposo G, Perez F TITLE Synchronization of secretory protein traffic in populations of cells. JOURNAL Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928. PUBMED 22406856 REFERENCE 2 (bases 1 to 8994) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8994 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 234..414 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 458..583 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1076..1309 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1493..1537 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1798..1915 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2005..2208 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2159..2179 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2205..2229 /label=LNCX /note="Human CMV promoter, forward primer" CDS 3049..3162 /codon_start=1 /product="streptavidin-binding peptide" /label=SBP /note="selected from a peptide library; binds streptavidin with nanomolar affinity (Keefe et al., 2001)" /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP" CDS 3172..3888 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3172..3888 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3217..3238) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3478..3497) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3825..3846 /label=EGFP-C /note="EGFP, forward primer" promoter 3964..4175 /label=EF-1-alpha core promoter /note="core promoter for human elongation factor EF-1-alpha" LTR 4188..4456 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from human T-cell leukemia virus (HTLV) type 1" CDS 4483..5082 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(4483..4502) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 4979..4999 /label=Puro-F /note="Puromycin resistance gene, forward primer" misc_feature 5089..5677 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5142..5162) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5560..5571) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 5751..5984 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 6056..6177 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6093..6112) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6147..6166 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 6196..6331 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6258..6277 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(6369..6385) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6369..6385) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6382..6404) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6393..6409 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6417..6447) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6462..6483 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6600..6617) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6771..7359) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6851..6870) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7530..8390) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8153..8172 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8391..8495) /gene="bla" /label=AmpR promoter primer_bind 8563..8581 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(8619..8641) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 8741..8760 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 8954..8976 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 8968..8985 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 8969..8985 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcaccggt ggatccggcg cgccatgtct accgagtcta tgattaggga cgtggaactg 2341 gctgaggagg cactgcccaa aaaaaccggc ggaccacagg gctctaggag atgtctgttt 2401 ctgtctctgt tctcttttct catcgtggct ggcgctacaa cactcttctg tctgctccat 2461 ttcggcgtga ttggaccaca gcgagaggaa tttccccggg atctgtcact catttcacca 2521 ctggcacagg ctgtccgatc ttcatctcgg actccatccg acaaacctgt cgcccatgtc 2581 gtcgccaacc cacaggccga gggccagctc cagtggctca ataggagggc aaacgctctg 2641 ctcgccaatg gcgtggaact ccgggataac cagctcgtcg tgcctagtga gggactgtac 2701 ctcatctact cccaggtgct gtttaagggc cagggatgtc cttctacaca tgtgctgctc 2761 acacacacaa tttcacggat cgccgtgtct taccagacta aagtcaatct gctctctgcc 2821 atcaaatccc catgtcagcg ggaaacacct gagggcgctg aggctaaacc ttggtacgaa 2881 cccatctacc tcggaggcgt gttccagctg gagaagggcg atagactgag tgctgagatc 2941 aatcgacccg actacctcga ctttgccgaa tctggccagg tctactttgg catcattgct 3001 ctgggcctcg agggccgagc tcatggcgca cctaggcctt tgaattccat ggacgagaag 3061 accactggtt ggcgaggtgg acacgttgtt gaaggactgg ctggggaact tgaacaactt 3121 cgtgcacgac tggagcatca cccacaaggt caacgtgaac cacctgcagg tatggtgagc 3181 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 3241 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 3301 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 3361 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac 3421 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 3481 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 3541 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 3601 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag 3661 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 3721 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 3781 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 3841 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggg ccggccttaa 3901 ggcctcgagg gccaattaat taagcggccg caaggatctg cgatcgctcc ggtgcccgtc 3961 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt 4021 gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc 4081 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg 4141 ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct 4201 ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc 4261 tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc 4321 tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc 4381 tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc 4441 tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga gtacaagccc 4501 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 4561 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 4621 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 4681 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 4741 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 4801 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 4861 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 4921 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 4981 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 5041 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa tcaacctctg 5101 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 5161 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 5221 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 5281 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 5341 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 5401 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 5461 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc 5521 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 5581 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 5641 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac 5701 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 5761 aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga 5821 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 5881 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 5941 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 6001 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 6061 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 6121 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 6181 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 6241 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 6301 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg 6361 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 6421 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 6481 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 6541 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 6601 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 6661 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 6721 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 6781 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 6841 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 6901 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 6961 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 7021 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 7081 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 7141 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 7201 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 7261 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 7321 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 7381 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 7441 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 7501 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 7561 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 7621 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 7681 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 7741 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 7801 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 7861 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 7921 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 7981 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 8041 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 8101 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 8161 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 8221 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 8281 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 8341 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 8401 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 8461 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 8521 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 8581 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 8641 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 8701 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta 8761 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 8821 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 8881 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta 8941 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg //