LOCUS Exported 3340 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION systhesis of vioC gene in the violacein pathway. ACCESSION . VERSION . KEYWORDS pKMV-VioC SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3340) AUTHORS Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J TITLE YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. JOURNAL Nucleic Acids Res. 2015 May 8. pii: gkv464. PUBMED 25956650 REFERENCE 2 (bases 1 to 3340) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3340 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 50..68 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind 77..94 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 78..94 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1444..1460) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1444..1460) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1472..1489) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1643..2231) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1723..1742) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2402..3217) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 3125..3144 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" promoter complement(3218..3322) /gene="bla" /label=AmpR promoter ORIGIN 1 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 61 cacgaggccc tttcgttgta aaacgacggc cagtcgtctc gatccgcagt gtcttgcgtc 121 tctgatgaaa agagctatca tcgttggtgg tggtttggct ggtggtttga ccgctatcta 181 cttggctaag agaggttacg aagttcacgt tgttgaaaag agaggtgacc cattgagaga 241 cttgtcttct tacgttgacg ttgtttctag tagagctatc ggtgtttcta tgaccgttag 301 aggtatcaag tctgttttgg ctgctggtat cccaagagct gaattggacg cttgtggtga 361 accaatcgtt gctatggctt tctctgttgg tggtcaatac agaatgagag aattgaagcc 421 attggaagac ttcagaccat tgtctttgaa cagagctgct ttccaaaagt tgttgaacaa 481 gtacgctaac ttggctggtg ttagatacta cttcgaacac aagtgtttgg acgttgactt 541 ggacggtaag tctgttttga tccaaggtaa ggacggtcaa ccacaaagat tgcaaggtga 601 catgatcatc ggtgctgacg gtgctcactc tgctgttaga caagctatgc aatctggttt 661 gagaagattc gagttccaac aaaccttctt cagacacggt tacaagacct tggttttgcc 721 agacgctcag gctttgggtt acagaaagga caccttgtac ttcttcggta tggactctgg 781 tggtttgttc gctggtagag ctgctaccat cccagacggt tctgtttcta tcgctgtttg 841 tttgccatac tctggttctc catctttgac caccaccgac gaaccaacca tgagagcttt 901 cttcgacaga tacttcggtg gtttgccacg cgacgctcgc gacgaaatgt tgagacaatt 961 cttggctaag ccatctaacg acttgatcaa cgttcgatct tctaccttcc actacaaggg 1021 taacgttttg ttgttgggtg acgctgctca cgctaccgct ccattcttgg gtcaaggtat 1081 gaacatggct ttggaagacg ctagaacctt cgttgaattg ttggacagac accaaggtga 1141 ccaagacaag gctttcccag agttcaccga attgagaaag gttcaagctg acgctatgca 1201 agacatggct agagctaact acgacgtttt gtcttgttct aacccaatct tcttcatgag 1261 agctagatac accagataca tgcactctaa gttcccaggt ttgtacccac cagacatggc 1321 tgaaaagttg tacttcacct ctgaaccata cgacagattg caacaaatcc aaagaaagca 1381 aaacgtttgg tacaagatcg gtagagttaa ctagcagaga cggagtcact gccaaccgag 1441 acggtcatag ctgtttcctg tgtgccgctt cctcgctcac tgactcgctg cgctcggtcg 1501 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta cccacagaat 1561 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 1621 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 1681 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 1741 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 1801 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 1861 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 1921 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 1981 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 2041 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 2101 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 2161 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 2221 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 2281 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 2341 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 2401 gttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa 2461 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc 2521 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac 2581 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga 2641 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc 2701 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt 2761 gcgcctgagc gagtcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg 2821 aatgcaaccg gcgcaggaac acggccagcg catcaacaat attttcacct gaatcaggat 2881 attcttctaa tacctggaat gctgttttcc cggggatcgc tgtggtgagt aaccatgcat 2941 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt 3001 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa 3061 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga 3121 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg 3181 gcctagagca agacgtttcc cgttgaatat ggctcatact cttccttttt caatattatt 3241 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 3301 ataaacaaat aggggttccg cgcacatttc cccgaaaagt //