LOCUS       Exported                 786 bp ds-DNA     linear   SYN 04-JUN-2021
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    GSK3beta (K85A)-pcw107-V5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 4, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(43..84)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    92..116
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(549..560)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 gtaatccaga ggttgattgt cgacttaacg cggtcattac tacgtagaat cgagaccgag
       61 gagagggtta gggataggct taccaccggt aaccactttg tacaagaaag ttggcaaggt
      121 ggagttggaa gctgatgcag aagcagcatt attggtctgt ccacggtctc cagtattagc
      181 atctgacgct gctgtggcat ttgtgggggt tgaagcagct gcttgaatcc gagcatgagg
      241 aggaataagg atggtagcca gaggtggatt acttgacagt tcttgagtgg tgaagttgaa
      301 gagtgcaggt gtgtctcgcc catttggtag tttgacattt gggtcccgta attcatcaaa
      361 aaatgaatgt gcacaagctt ccagtggtgt tagtcgggca gttggtgtat actccagcag
      421 acggctacac agtgcaattg cctccggtgg agttcggggt cggaagacct tagtccaagg
      481 atgtgcctta atttgaggga atttaaattc tgtgtagttt gggttcattt ctctgatttg
      541 ctcccttgtt ggagttccca ggaccttgat tatttctacc aactgatcca caccactatc
      601 ccctggaaat attggttgtc ctagtaacag ctcagccaac acacagccag cagaccatac
      661 atctatacta gaggtataat cagtggctcc aaagatcaac tctggtgccc tatagtaccg
      721 agaacagata tacgaaacat tgggttctcc tcggaccagc tgctttgcac ttccaaagtc
      781 acagag
//
