LOCUS       Exported                 754 bp ds-DNA     linear   SYN 25-MAY-2021
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    FLAG-MKK6 (S207E,T211E)-pcw107
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 25, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(43..84)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    92..116
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtaatccaga ggttgattgt cgacttaacg cggtcattac tacgtagaat cgagaccgag
       61 gagagggtta gggataggct taccaccggt aaccactttg tacaagaaag ttggcaatta
      121 gtctccaaga atcagtttta caaaagatgc cacatctgtt cctttggatt catgtagggt
      181 gaaaaatgga tgttgcatta gctctgggta tgtaggccgt tctttggaat tcttctttaa
      241 gcactgtgag gtaaagtcaa caaactctgc agagaacttg tctgctggga gttgtggcga
      301 tggctcctct accacctgtt tgagctgctg aaatggagtt ccccatgaat catagggaaa
      361 tcgaaggatg gccaactcaa tcatcgtgat gcccagactc caaatgtcag acttcacact
      421 gtatcccttc tggttgagct ctgggtttat tctttcaggg gccatgtatg gtttgcaacc
      481 tgcatcaatt tctttagcaa cctcgtccac caagtagcca ctgattccaa aatcgcacat
      541 cttcacttga ccgagagcat tgatgagtac attagaaggc ttgacgtctc tgtgaatgac
      601 agacagctta ctatgtaaat gttctaatgc ttttacaata gaaactgcta ttttccctaa
      661 gatgtcctct ggaattgtct ggcctttatc aataacttgt ttgtagaatt tatctagtga
      721 tgtatccatg agctccatgc agatccacac atca
//
