LOCUS Exported 718 bp ds-DNA linear SYN 22-MAY-2021 DEFINITION when used to produce lentivirus, express physiological levels of insert . ACCESSION . VERSION . KEYWORDS SmoM2 (W535L)-pcw107-V5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 718) AUTHORS Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN, Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, McDonnell DP, Sabatini DM, Wood KC TITLE Systematic identification of signaling pathways with potential to confer anticancer drug resistance. JOURNAL Sci Signal. 2014 Dec 23;7(357):ra121. doi: 10.1126/scisignal.aaa1877. PUBMED 25538079 REFERENCE 2 (bases 1 to 718) AUTHORS . TITLE Direct Submission JOURNAL Exported May 22, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..718 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(36..77) /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" protein_bind 85..109 /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" ORIGIN 1 agaggttgat tgtcgactta acgcggtcat tactacgtag aatcgagacc gaggagaggg 61 ttagggatag gcttaccacc ggtaaccact ttgtacaaga aagttggcaa gaagtccgag 121 tctgcatcca tgagttctgt gtccatcagg ttggtgcggg agtgaatagg ccccaggccc 181 tgtcggcggc catgagccca tgccacgggg gccggtgcac tgggcagaaa tggatcctga 241 gggggactgg gctctgggca gaatgggttg gagaccaggg tccacgctcc ctgtcggcaa 301 gagtccccag ctccccaggc acctgcagcc accaggcatt tctgccgggg cagctgaggc 361 agtcgaggaa tggtactggg ggcaggggca ggggggtgaa gctcaggggg cggcgccagc 421 gggcacacct ccttcttcct cttcctcctc ttcttcttcc ggcccaggcg cttctgcagc 481 tctggggaga tctctgcctc aaccagccac aggttggctt gttcctctgg gggcactgga 541 gttgccacag gggtgacaga aatatcctgg ggcagtatgg ctcctctccg agccaccatc 601 ttggtgacat gctgggccca ggcagaggag acatcagctg agggctcatt gaggtcaaag 661 gccaagcccg ccacgggccc gtcgtgggac acagtgtgca tgctgaagga cagctcct //