LOCUS       Exported                 658 bp ds-DNA     linear   SYN 14-JUN-2021
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    BCL2-pcw107-V5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 14, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(39..80)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    88..112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tccagaggtt gattgtcgac ttaacgcggt cattactacg tagaatcgag accgaggaga
       61 gggttaggga taggcttacc accggtaacc actttgtaca agaaagttgg caacttgtgg
      121 cccagatagg cacccagggt gatgcaagct cccaccaggg ccaaactgag cagagtcttc
      181 agagacagcc aggagaaatc aaacagaggc cgcatgctgg ggccgtacag ttccacaaag
      241 gcatcccagc ctccgttatc ctggatccag gtgtgcaggt gccggttcag gtactcagtc
      301 atccacaggg cgatgttgtc caccaggggc gacatctccc ggttgacgct ctccacacac
      361 atgaccccac cgaactcaaa gaaggccaca atcctccccc agttcacccc gtccctgaag
      421 agctcctcca ccaccgtggc aaagcgtccc cgcgcggtga agggcgtcag gtgcagctgg
      481 ctggacatct cggcgaagtc gcggcggtag cggcgggaga agtcgtcgcc ggcctggcgg
      541 agggtcaggt ggaccacagg tggcaccggg ctgagcgcag gccccgcggc ggcgccgggg
      601 gcagccgggg tctgcagcgg cgaggtcctg gcgaccgggt cccgggatgc ggctggat
//
