LOCUS       Exported                 725 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    FLAG-YAP2 (8SA)-pcw107
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             86..109
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tcaccgacct ctctccccag gggctagcat cgatggatca acaagtttgt acaaaaaagt
       61 tggcggaagc ggcggcggca ccatggacta caaagacgat gacgataaag caaggctcga
      121 atcggtacct aaggatcccg ggcagcagcc gccgcctcaa ccggcccccc agggccaagg
      181 gcagccgcct tcgcagcccc cgcaggggca gggcccgccg tccggacccg ggcaaccggc
      241 acccgcggcg acccaggcgg cgccgcaggc accccccgcc gggcatcaga tcgtgcacgt
      301 ccgcggggac gcggagaccg acctggaggc gctcttcaac gccgtcatga accccaagac
      361 ggccaacgtg ccccagaccg tgcccatgag gctccggaag ctgcccgact ccttcttcaa
      421 gccgccggag cccaaatccc actcccgaca ggccgctact gatgcaggca ctgcaggagc
      481 cctgactcca cagcatgttc gagctcatgc cgctccagct gctctgcagt tgggagctgt
      541 ttctcctggg acactgaccc ccactggagt agtctctggc ccagcagcta cacccacagc
      601 tcagcatctt cgacaggctg cttttgagat acctgatgat gtacctctgc cagcaggttg
      661 ggagatggca aagacatctt ctggtcagag atacttctta aatcacatcg atcagacaac
      721 aacat
//
