LOCUS       Exported                 600 bp ds-DNA     linear   SYN 29-MAY-2021
DEFINITION  3rd generation lentiviral transfer plasmid. When used to produce 
            lentivirus, express physiological levels of insert .
ACCESSION   .
VERSION     .
KEYWORDS    p53 (dominant negative R175H mutant)-pcw107-V5
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 29, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(44..85)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    93..117
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtaatccaga gggttgattg tcgacttaac gcggtcatta ctacgtagaa tcgagaccga
       61 ggagagggtt agggataggc ttaccaccgg taaccacttt gtacaagaaa gttggcaagt
      121 ctgagtcagg cccttctgtc ttgaacatga gttttttatg gcgggaggta gactgaccct
      181 ttttggactt caggtggctg gagtgagccc tgctcccccc cggctccttc ccagcctggg
      241 catccttgag ttccaaggcc tcattcagct ctcggaacat ctcgaagcgc tcacgcccac
      301 ggatctgaag ggtgaaatat tctccatcca gtggtttctt ctttggctgg ggagaggagc
      361 tggtgttgtt gggcagtgct cgcttagtgc tccctggggg cagctcgtgg tgaggctccc
      421 ctttcttgcg gagattctct tcctctgtgc gccggtctct cccaggacag gcacaaacac
      481 gcacctcaaa gctgttccgt cccagtagat taccactgga gtcttccagt gtgatgatgg
      541 tgaggatggg cctccggttc atgccgccca tgcaggaact gttacacatg tagttgtagt
//
