LOCUS Exported 12227 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter vector to select iChon cells. ACCESSION . VERSION . KEYWORDS pLe6-Delta -hLP-mcs/(EGFP-IresPuro-hInt) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12227) AUTHORS Outani H, Okada M, Yamashita A, Nakagawa K, Yoshikawa H, Tsumaki N TITLE Direct induction of chondrogenic cells from human dermal fibroblast culture by defined factors. JOURNAL PLoS One. 2013 Oct 16;8(10):e77365. doi: 10.1371/journal.pone.0077365. eCollection 2013. PUBMED 24146984 REFERENCE 2 (bases 1 to 12227) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12227 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 3..229 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter" LTR 230..410 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 457..582 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1075..1308 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 1493..1537 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 1804..1921 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" protein_bind 1951..1971 /gene="mutant version of attB" /label=attB4 /bound_moiety="BP Clonase(TM)" /note="core recombination site for the Gateway(R) BP reaction" protein_bind 3180..3204 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" regulatory 3242..3251 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3248..3967 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" CDS 3248..3964 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3293..3314) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3554..3573) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3901..3922 /label=EGFP-C /note="EGFP, forward primer" misc_feature 3994..4580 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4161..4178) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 4388..4407 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 4581..5183 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind 5080..5100 /label=Puro-F /note="Puromycin resistance gene, forward primer" protein_bind complement(7938..7962) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 8015..8056 /codon_start=1 /product="epitope tag from simian virus 5" /label=V5 tag /translation="GKPIPNPLLGLDST" misc_feature 8080..8668 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(8133..8153) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(8551..8562) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 8748..8981 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" polyA_signal 9053..9174 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(9090..9109) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 9144..9163 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin 9214..9349 /label=SV40 ori /note="SV40 origin of replication" primer_bind 9276..9295 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(9369..9388) /label=T7 /note="T7 promoter, forward primer" promoter complement(9370..9388) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(9398..9415) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(9398..9414) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9407..9429) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 9556..10011 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9643..9662) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 9853..9874 /label=F1ori-F /note="F1 origin, forward primer" promoter 10037..10141 /gene="bla" /label=AmpR promoter CDS 10142..11002 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(10360..10379) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 11173..11761 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 11662..11681 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 11915..11932 /label=L4440 /note="L4440 vector, forward primer" protein_bind 12049..12070 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 12085..12115 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 12123..12139 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 12128..12150 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 12147..12163 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 12147..12163 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 12182..12202 /label=T3 /note="T3 promoter, forward primer" promoter 12184..12202 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" ORIGIN 1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca 61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga 121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt 181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg 241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg 361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg 421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt 481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga 961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct 1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatgtc gacgttaacg ctagtgatat caactttgta tagaaaagtt ggctccgaat 1981 tcaatcgctg ttttctggac tgaggtgact gtatgaggat gggcagcgcc gtttcgggct 2041 tggggggggt atccggttgg gagctccgga cgcgatccct ggacgccgcc gccgaggttc 2101 catggggccc aagaggtgca cgcagggtgg gggacacaag gcaagctttg tcggggaggg 2161 ggggagaggg tgtgccaggc tgggggcggg gccggccgga ggaggaaggg ggggcggtgg 2221 attctcaaag gcgccttgtt cgctcgtgcc ctctccgcgc cagcgggcgg cggcgcctcg 2281 gctcgctgcg gccttccctc cgggcgcgct gcgggctccg ggcagacccg gcgccgtgcc 2341 cgctcgtggg ggcgcactgg gcgccgagcg ctgcgtgctt ctcaggcgtt gcccgcccgg 2401 aggcggccca cgtccccgag tgaccccatt tccctggacc cttccaacca gagtcgactg 2461 ctcgctcatc ttaccactgc tccccctcta cctcggctct gggactccct aaccgcgtcc 2521 cccatttgca tctccttgga cctgactatc ctgtgtttgt cggtgggtcc cagtctctgg 2581 cctcttgctc tccgtagaac accttccccc acatcccccg ccccagttcg tcgctatcta 2641 tagccttgtc tataaatacc cccgccccgg cccggctctg taattacacg gggcggggtg 2701 agggaagtaa ttatggagac ctgattatgg gtggggtggg gactgcgacc cccaggcccg 2761 cctctccccc tcccactgtg ccctaaatcc cgcccaggcc tctgctgaaa gggggctctg 2821 ggcccccaag agggagggaa tgggagggag tgtgtgtgac tggacgtttg ggtcctagaa 2881 aaggaagggg ctagggaaga tattggggtt cccgaaaaga gaatcttagg gtacaggccg 2941 ttgagaccta caaggggcag gagagagcga gcgatagagg gagggttccc gcctccctcc 3001 ccaggtggag actgagggtg gggtttccct tcggtggctg tgggcgggcg gctggaggcg 3061 ggggccgggc cgggggcggg ggcgaggtgg gggctctggg cgccagggtg gccggggaca 3121 cacagaagcg gcagccaccg aggagggagc agtgccggga gctcgagtgt caattcgacc 3181 caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg atccaccggt 3241 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 3301 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 3361 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 3421 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 3481 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 3541 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 3601 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 3661 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 3721 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 3781 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 3841 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 3901 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 3961 caagtaaagc ggccgcaagc tgagcaggat ccgcccctct ccctcccccc cccctaacgt 4021 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac 4081 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag 4141 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa 4201 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag 4261 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga 4321 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag 4381 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc 4441 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag 4501 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg 4561 atgataatat ggccacaacc atggcaaccg agtacaagcc cacggtgcgc ctcgccaccc 4621 gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca 4681 cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg caagaactct 4741 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg 4801 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc 4861 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc 4921 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg 4981 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc 5041 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc 5101 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca 5161 tgacccgcaa gcccggtgcc tgagcggccg caactagtcc tcgaggtaag aggagtcctg 5221 atgcctgggt cctcggagtc aggctggagc tctgagtgtc tctgggaagg ggccagctga 5281 tgcctggggc agcagcttct gagtctaaca aggagatttg gggcttcaag gctacaggct 5341 ggagggcagg acacctgtgt tccttggcat cagggtagga atctctttgt ccttgaagtg 5401 actgggggag ggcagatagg gctgaaaggt ggaggaccac tttagagacc ctgaatgggg 5461 atggatgctt gtcttgagtc cttgggaaga gctgaagatg gccagggcca gcccggtcca 5521 gtcattgctg gtgtggggat ggtagacgcc aatgttgatg ggtgaggcgt gtgtgtctta 5581 tggcctttct gtcgatatct ctgggataag gggtgagcac ctgtgattca gagcagagcc 5641 cagtaagccc agaaagaaag gaagccttga ctacctgtct cattggcctc ctggacaggg 5701 tccccctccc cctccctgtg ccctcgatgc tgccacgcag ctggctctgg ggagcactgg 5761 ggctggctgc caacaggacg gccctccgga caggccagga tcaggtgtct gaggccagag 5821 ccaactcttt gcattcctgc ctagcccctc ctccctctgg ccagccaggc tcccctggga 5881 ccctggtgcc cacaaccctg atcttttcct tctttttccc aggacccaga attcctggct 5941 tctaggaaag gggatttgat gtcaaggagg gggctgtgag caccaggtcc tcagcaggct 6001 ggaaaaagcc catttctgga gacactgagg gctgatttat atttaacttg actattcaat 6061 tcttctctgc tttagagaga aaatggtcag aatggcagca caaaagattt agattagatt 6121 acagaaggaa gaacttccaa gatgtgagga cagtgaagcc cagggccagg aaattgaggc 6181 gtatggtgcc tgtagactct aggagtcttt cttaggggga agagggatcc tctggcatgg 6241 aggcaggggg atggctaaga tgactgtttc ttatttcagg ggctgagtgg gtagagatgg 6301 gactttaggg aaatgagtta ctgtagaatt cctaaggtta gagaataagg catttggggg 6361 gccagaggta gagtggtgga gggtaatggg gctaccagtg gagagcctgt ggggcagatg 6421 aggctggata caggggggca agtatgtggc ctggtgtggg aagatggtgg gcggacctgt 6481 gggtaggttg tccatatgat cgaattgtac tcccttggga tttggtggag ggtggggacc 6541 tccgaggtct ccttggcctg gggtctctgt gagtctctgt gtctctgtct cactcaccct 6601 tcgtctctca aagcggccct tgtgtgttgg tgtttgcgga gctgccacac gcgcaggggc 6661 caggcttagg tgggggttgg gggggaaggc cgtggtttcc cggctcctgg actcaaaggg 6721 ccttttctct gcctgcccgc cccacctcac ccaccccacc cagctccact ctcctgattg 6781 ctctggcctc tgccctgccc cacacttcta tgaaagtttt gctggaactc tctttgggat 6841 ttttccaatc tggagctgat ggtgtgaaga gtatgtgtat aggggagcaa gtgagggcaa 6901 ggtgatcttt tgttttccag ggagtttgag ccctaggaag ctgagttcta gttagacagg 6961 gctggtgggc tggatcttga atctttgggt cccaggtggt ggggttggtg ggggtggtga 7021 gacaggattg aggttccagg tgtggggagc ttgcctgggt tctgggcaag cactctgatg 7081 ggatccacag atgtgtggct cagggccctg gacctgttcc ggctcctcgt ggcagcttca 7141 gcccctgccc cccattcctc cacccctccc caactcatct cctaaaccag gaggcacaga 7201 ctcctgggag agctttcttg tatctttttc ctcagcatgc tcagggttgt tcccacccca 7261 tcccccaaac ctgggatctg tagctgcttg aagcccctct catagagctg cttctctgat 7321 ctcttcctcg tggcccccct gcacctcctg tgtccaggat gatcgattgc ccagatgttg 7381 tttgtgctgg agacgggaca gatgccagag acccagagag tgagagatat ggaaagagag 7441 tgagagcgag ggagagaaaa agatggacag aatgtgagag aaatggagga agacagaaga 7501 gacagagaca gaaagaccaa cataaagaga cagagagagt aagagagtga gagagacagg 7561 agacagatga agagttgaga gaagtattca gagagtacag agagagacag attcataagc 7621 taaaagacaa agagagagcg taagagaggc agatgctagg agagacaggg gcaggagtgt 7681 catggggttg aatgccagct gatgctgccc tgcctccagg tacctttgca tttcccatcc 7741 attcaggtac tgttcagaag caagtgaggg tgaggtgacc tttcccttgc agggagtttg 7801 agccctggaa agctgagttc tagatcccaa atgtccccta tttatgccct cctgagggca 7861 tgtcccttct cctgataatc atggactctc ccagagatct ggaagcttgg gaattcggca 7921 tatgtcgaga tatctagacc cagctttctt gtacaaagtg gttgatatcc agcacagtgg 7981 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct 8041 cggtctcgat tctacgcgta ccggttagta atgatcgaca atcaacctct ggattacaaa 8101 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac 8161 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc 8221 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt 8281 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc 8341 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc 8401 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg 8461 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt 8521 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc 8581 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt 8641 cggatctccc tttgggccgc ctccccgcct ggcgatggta cctttaagac caatgactta 8701 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 8761 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca 8821 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 8881 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 8941 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 9001 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 9061 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 9121 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 9181 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 9241 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 9301 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac 9361 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc 9421 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg 9481 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc 9541 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 9601 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 9661 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 9721 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 9781 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 9841 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 9901 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 9961 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac 10021 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 10081 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 10141 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc 10201 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc 10261 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc 10321 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc 10381 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt 10441 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt 10501 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat 10561 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct 10621 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat 10681 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc 10741 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg 10801 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc 10861 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta 10921 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc 10981 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga 11041 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat 11101 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat 11161 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 11221 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 11281 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 11341 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 11401 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 11461 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 11521 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 11581 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 11641 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 11701 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 11761 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 11821 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 11881 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 11941 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 12001 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 12061 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg 12121 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg 12181 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagctt //