LOCUS Exported 15606 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.. ACCESSION . VERSION . KEYWORDS pFastBac-GFP-dynein-2(D1091–Q4307) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 15606) AUTHORS Schmidt H, Zalyte R, Urnavicius L, Carter AP TITLE Structure of human cytoplasmic dynein-2 primed for its power stroke. JOURNAL Nature. 2015 Feb 19;518(7539):435-8. doi: 10.1038/nature14023. Epub 2014 Dec 1. PUBMED 25470043 REFERENCE 2 (bases 1 to 15606) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..15606 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 2..457 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(89..108) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 299..320 /label=F1ori-F /note="F1 origin, forward primer" promoter 484..588 /gene="bla" /label=AmpR promoter CDS 589..1449 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(807..826) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1620..2208 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2109..2128 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2362..2379 /label=L4440 /note="L4440 vector, forward primer" mobile_element 2511..2735 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (right end of the Tn7 transposon)" CDS complement(2802..3335) /codon_start=1 /gene="aacC1" /product="gentamycin acetyltransferase" /label=GmR /note="confers resistance to gentamycin" /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR EEVMHFDIDPSTAT" promoter complement(3524..3552) /gene="intI1 (promoter lies within the coding sequence)" /label=Pc promoter /note="class 1 integron promoter" promoter 3904..3995 /gene="polh from Autographa californica" /label=polyhedrin promoter /note="promoter for the baculovirus polyhedrin gene" primer_bind 3920..3937 /label=Polyhedrin-F /note="Polyhedrin promoter, forward primer" CDS 4062..4085 /codon_start=1 /product="8xHis affinity tag" /label=8xHis /translation="HHHHHHHH" CDS 4128..4301 /codon_start=1 /product="IgG-binding unit of Staphylococcus aureus protein A" /label=ProtA /translation="VDNKFNKEQQNAYYEILHLPNLNEGQRNAFIQSLKDDPSQSANLL AEAKKLNDAQAPK" CDS 4305..4475 /codon_start=1 /product="IgG-binding unit of Staphylococcus aureus protein A" /label=ProtA /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA EAEKLNDAQAPK" CDS 4533..4553 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" CDS 4575..4595 /codon_start=1 /product="tobacco etch virus (TEV) protease recognition and cleavage site" /label=TEV site /translation="ENLYFQG" polyA_signal 15096..15230 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(15133..15152) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 15187..15206 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" mobile_element 15259..15424 /mobile_element_type="transposon:Tn7" /note="mini-Tn7 element (left end of the Tn7 transposon)" ORIGIN 1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc 61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc 121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt 181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg 241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt 301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta 361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt 421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat 481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg 2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg 2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct 2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga 2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag 2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt 2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga 2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac 2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg 2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg 2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca 2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact 3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc 3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta 3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct 3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg 3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg 3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca 3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa 3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa 3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca 3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac 3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc 3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg 3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt 3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt 3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa 3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacc 4081 atcacggttc tatgtgcatg gcaggccttg cgcaacacga tgaagccgtg gacaacaaat 4141 tcaacaaaga acaacaaaac gcgtactatg agatcttaca tttacctaac ttaaacgaag 4201 gacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt 4261 tagcagaagc taaaaagcta aatgatgctc aggcgccgaa agtagacaac aaattcaaca 4321 aagaacaaca aaacgcgttc tatgagatct tacatttacc taacttaaac gaagaacaac 4381 gaaacgcctt catccaaagt ttaaaagatg acccaagcca aagcgctaac cttttagcag 4441 aagctgaaaa gctaaatgat gctcaggcgc cgaaagtaga cgcaaattcc gcggggaagt 4501 caaccgatta tgatattcca actactgcta gcgagaacct atatttccag ggagattatg 4561 atatccctac gactgagaat ctttattttc agggcatgtc taaaggtgaa gaattattca 4621 ctggtgttgt cccaattttg gttgaattag atggtgatgt taatggtcac aaattttctg 4681 tctccggtga aggtgaaggt gatgctactt acggtaaatt gaccttaaaa tttatttgta 4741 ctactggtaa attgccagtt ccatggccaa ccttagtcac tactttcggt tatggtgttc 4801 aatgttttgc gagataccca gatcatatga aacaacatga ctttttcaag tctgccatgc 4861 cagaaggtta tgttcaagaa agaactattt ttttcaaaga tgacggtaac tacaagacca 4921 gagctgaagt caagtttgaa ggtgatacct tagttaatag aatcgaatta aaaggtattg 4981 attttaaaga agatggtaac attttaggtc acaaattgga atacaactat aactctcaca 5041 atgtttacat catggctgac aaacaaaaga atggtatcaa agttaacttc aaaattagac 5101 acaacattga agacggttct gttcaattag ctgaccatta tcaacaaaat actccaattg 5161 gtgatggtcc agtcttgtta ccagacaacc attacttatc cactcaatct gccttatcca 5221 aggatccaaa cgaaaagaga gaccacatgg tcttgttaga atttgttact gctgctggta 5281 ttaccggttc tgacgatttg gaagtgacac gcaagaagct ggtcgatgac tgtcaccact 5341 tccgcctcga agaacctaac ttctctctcg cctccagcat cagcaaggat attgagtcct 5401 gtgctcagat ttgggccttc tacgaagagt tccagcaggg attccaagaa atggccaatg 5461 aagattggat cacctttcgc actaagacct acttgtttga ggagtttctg atgaattggc 5521 acgaccgcct caggaaagtg gaggagcatt ctgtgatgac tgtcaagctc caatctgagg 5581 tggacaaata taagattgtt atccctatcc tgaagtacgt ccgcggagaa cacctgtcac 5641 ccgatcactg gctggatctg ttccgcttgc tgggtctgcc tcgcggcaca tctctggaga 5701 aactgctgtt cggtgacctg ctgagagttg ccgataccat cgtggccaag gctgctgacc 5761 tgaaagatct gaactcacgc gcccagggtg aagtgaccat ccgcgaagca ctcagggaac 5821 tggatttgtg gggcgtgggt gctgtgttca cactgatcga ctatgaggac tcccagagcc 5881 gcaccatgaa gctgatcaag gattggaagg acatcgtcaa ccaggtgggc gacaatagat 5941 gcctcctgca gtccttgaag gactcaccat actataaagg ctttgaagac aaggtcagca 6001 tctgggaaag gaaactcgcc gaactggacg aatatttgca gaacctcaac catattcaga 6061 gaaagtgggt ttacctcgaa ccaatctttg gtcgcggagc cctgcccaaa gagcagacca 6121 gattcaacag ggtggatgaa gatttccgca gcatcatgac agatatcaag aaggacaatc 6181 gcgtcacaac cttgactacc cacgcaggca ttcgcaactc actgctgacc atcctggacc 6241 aattgcagag atgccagcgc agcctcaacg agttcctgga ggagaagcgc agcgccttcc 6301 ctcgcttcta cttcatcgga gacgatgacc tgctggagat cttgggccag tcaaccaatc 6361 catccgtgat tcagtctcac ctcaagaagc tgtttgctgg tatcaactct gtctgtttcg 6421 atgagaagtc taagcacatt actgcaatga agtccttgga gggagaagtt gtgccattca 6481 agaataaggt gcccttgtcc aataacgtcg aaacctggct gaacgatctg gccctggaga 6541 tgaagaagac cctggagcag ctgctgaagg agtgcgtgac aaccggacgc agctctcagg 6601 gagctgtgga cccttctctg ttcccatcac agatcctgtg cttggccgaa cagatcaagt 6661 ttaccgaaga tgtggagaac gcaattaaag atcactccct gcaccagatt gagacacagc 6721 tggtgaacaa attggagcag tatactaaca tcgacacatc ttccgaggac ccaggtaaca 6781 cagagtccgg tattctggag ctgaaactga aagcactgat tctcgacatt atccataaca 6841 tcgacgtggt caagcagctg aaccaaatcc aagtgcacac caccgaagat tgggcctgga 6901 agaagcagtt gaggttctac atgaagtccg accacacctg ttgcgttcag atggttgaca 6961 gcgagttcca gtacacctat gagtaccaag gaaatgccag caagctcgtt tacactccac 7021 tcactgacaa gtgttacctc accttgacac aggctatgaa gatgggcctg ggaggcaacc 7081 catacggtcc agctggcact ggtaagacag agagcgttaa ggcactcgga ggtctgctgg 7141 gcaggcaggt cctcgtgttc aactgtgatg aaggaatcga cgttaagtcc atgggaagaa 7201 tctttgttgg cctcgttaag tgtggagctt ggggttgctt cgacgagttc aacaggctgg 7261 aggaatctgt gctgagcgcc gtctctatgc agatccagac catccaggac gcattgaaga 7321 accacaggac cgtctgcgag ctgttgggta aggaagtgga ggtgaactcc aactccggaa 7381 tcttcatcac aatgaatccc gcaggtaaag gatatggagg aagacagaaa ctcccagaca 7441 acctgaagca gctgttccgc ccagtggcta tgtcccatcc agacaatgag ctgatcgccg 7501 aagtcatcct ctattccgag ggattcaaag atgctaaagt tctctccaga aagctcgtgg 7561 ccatcttcaa tctgtcaaga gaactcctga cacctcagca gcattacgac tggggtctga 7621 gagccctcaa gaccgtcctg agaggttcag gaaatctcct caggcagctg aacaagagcg 7681 gtacaacaca gaatgcaaat gagagccaca ttgtcgtcca ggctctgagg ctgaatacca 7741 tgtcaaagtt cacattcaca gactgcacaa gatttgacgc tctgattaaa gatgtgttcc 7801 ctggtattga actcaaagaa gtggagtatg acgagctgag cgccgctttg aagcaggtgt 7861 ttgaggaggc taactatgag attatcccta atcagatcaa gaaagcattg gaactgtatg 7921 aacagctgtg tcagaggatg ggagtggtga ttgtgggccc atcaggcgca ggtaagagca 7981 ctctctggag aatgctgaga gcagcactgt gcaagactgg aaaggtggtg aagcaataca 8041 ccatgaatcc caaggccatg cccaggtacc aactgctggg ccatatcgac atggacacca 8101 gagaatggag cgacggcgtg ctcacaaact ccgccagaca agtcgtgcgc gaacctcaag 8161 acgtcagctc ttggatcatc tgcgatggtg atattgaccc tgagtggatc gagtccctga 8221 attccgtgtt ggatgacaac aggctcctca caatgccttc tggtgagaga atccagttcg 8281 gtcctaacgt gaacttcgtg ttcgagacac acgatctcag ctgtgctagc ccagctacta 8341 tctcccgcat gggaatgatc ttcttgtccg acgaggagac agatttgaac tcattgatca 8401 agtcttggct cagaaaccag cctgcagaat ataggaataa cctggagaac tggatcggtg 8461 attacttcga gaaggctttg cagtgggtgc tgaaacagaa cgactatgtc gtcgaaacca 8521 gcctggtcgg tacagttatg aacggactct cccatctgca cggatgcaga gatcacgatg 8581 agtttatcat caatttgatc cgcggactgg gaggtaactt gaatatgaaa tctcgcctgg 8641 agttcactaa agaagtgttc cactgggcta gggagtcacc acctgacttc cacaaaccta 8701 tggacaccta ctatgattcc acaagaggca ggttggccac ctacgtgctg aagaagcctg 8761 aggacctcac cgctgacgac ttctccaacg gactgactct gcccgtgatc cagactccag 8821 acatgcagcg cggactcgat tactttaagc cctggctcag ctccgatacc aagcaacctt 8881 tcattctcgt gggaccagag ggatgtggta aaggaatgct cctgaggtat gcattctccc 8941 agctccgctc aacccaaatt gccactgttc actgttcagc ccaaacaact tcaaggcatc 9001 tcctccagaa gctcagccag acctgtatgg ttatcagcac caacaccggc agagtttacc 9061 gcccaaagga ttgtgagcgc ctcgtgttgt acctcaaaga tatcaatctg cccaaactcg 9121 ataagtgggg cacttccacc ctcgtggcat ttctccaaca ggtgctgacc taccagggct 9181 tctacgacga gaacctggag tgggtcggat tggagaacat ccagattgtg gcttccatgt 9241 ctgccggcgg taggttggga aggcataagc tcaccaccag gtttacatca attgtgagac 9301 tgtgctcaat cgattatccc gagcgcgaac agttgcagac catctatggc gcctacctcg 9361 aacctgtcct ccacaagaat ttgaagaacc atagcatctg gggctcatca agcaagatct 9421 acctcttggc tggctctatg gttcaggtgt acgaacaagt ccgcgccaag ttcaccgtcg 9481 atgattactc acattacttc ttcacaccct gcattctgac acaatgggtt ctgggactgt 9541 ttcgctatga cctggaggga ggctcatcaa accacccatt ggattatgtc ctcgaaatcg 9601 tggcttacga agcccgcagg ctctttagag ataagattgt tggcgctaag gaactgcatc 9661 tgttcgatat tatcctcacc tctgtgtttc aaggtgattg gggctctgat atcctggata 9721 atatgtccga ttccttctat gtgacatggg gagccaggca caactccggt gcaagggctg 9781 ctccaggcca accattgcca cctcatggca agcctctcgg caaactgaac tcaactgact 9841 tgaaggacgt gatcaagaag ggcctgattc actacggccg cgacaaccag aacctggaca 9901 ttctgttgtt ccacgaggtc ctggagtata tgtccagaat tgatcgcgtc ctgtctttcc 9961 ctggtggatc actgctgctg gccggacgct ctggagttgg aagacgcact attacttctc 10021 tggtcagcca tatgcatgga gccgtcctgt tctctccaaa gatcagcaga ggctacgaac 10081 tcaagcaatt caagaacgat ctgaaacacg tcttgcaact cgccggtatc gaggcccagc 10141 aggtcgtcct cctcttggag gactatcaat tcgtccaccc aaccttcctg gagatgatca 10201 actccctgct gtcctctggc gaggtgcccg gcttgtacac tttggaggaa ctggagccac 10261 tgctcctccc attgaaggat caggcatcac aggacggctt cttcggccca gtgttcaatt 10321 acttcaccta tcgcattcaa cagaatctcc acattgtgct gattatggac agcgctaatt 10381 ccaatttcat gatcaattgc gagagcaatc ccgccctcca taagaagtgc caggtcctct 10441 ggatggaggg ttggtctaat tcttctatga agaagattcc cgagatgttg tttagcgaga 10501 ctggaggcgg tgagaagtac aacgacaaga agcgcaaaga ggagaagaag aagaactccg 10561 tcgatcctga tttcctcaag agcttcctgc tgatccacga gtcttgcaaa gcttacggag 10621 ctactcctag ccagtacatg accttcctcc acgtctattc cgccatctcc agctcaaaga 10681 agaaggagct gctgaagcgc caatctcatc tgcaggccgg agtcagcaag ctgaacgaag 10741 ccaaagctct ggtggatgaa ctgaatcgca aggctggcga acaatcagtc ctcctgaaga 10801 ctaagcagga tgaagctgac gctgccctgc aaatgattac cgtgtctatg caggatgctt 10861 ccgagcagaa gacagagctg gagaggctga agcaccgcat cgcagaggag gtggtcaaga 10921 tcgaggagag aaagaacaag attgacgacg aactcaaaga ggtgcagcct ctggtgaacg 10981 aggccaagct cgccgtgggt aatatcaaac cagagtctct ctcagagatc aggtcactga 11041 gaatgccacc agacgttatc cgcgacatcc tggagggcgt cctgcgcttg atgggtatct 11101 ttgacacctc ttgggtgtct atgaagtctt tcttggccaa gcgcggtgtc agggaggaca 11161 ttgctacttt cgacgctagg aacatctcca aggaaattag ggaatctgtg gaggaactgc 11221 tgttcaagaa taaaggttca tttgatccca agaacgctaa gagagcatca actgcagctg 11281 cacccttggc agcctgggtt aaggccaata tccagtactc tcacgtgctc gaacgcatcc 11341 accctctgga gactgagcaa gccggcctgg aaagcaacct gaagaagacc gaggatagaa 11401 agaggaaact ggaagaactc ctcaattctg tcggtcagaa ggtgtcagaa ctgaaggaga 11461 aatttcagag caggacctca gaagcagcta agttggaagc tgaggtgtcc aaggctcagg 11521 agactatcaa agcagctgaa gtgttgatta atcagctgga ccgcgaacac aagagatgga 11581 atgctcaggt cgtcgagatt actgaggagc tggcaaccct cccaaagagg gctcagttgg 11641 ccgcagcctt tatcacctac ttgtccgctg cacctgagtc tctcagaaag acatgtctgg 11701 aggagtggac caagtctgcc ggcctggaga agtttgacct gagaagattt ctctgcaccg 11761 agtcagagca gctgatctgg aagtctgaag gtctgcccag cgatgacctc tcaatcgaga 11821 acgcactggt tatcttgcaa tcccgcgttt gccctttcct catcgatccc agctcacagg 11881 ctactgagtg gctgaagact cacttgaagg attccaggct ggaagtgatc aaccagcaag 11941 actccaactt catcactgcc ctggaactcg ccgtccgctt cggcaagacc ttgatcatcc 12001 aggagatgga tggcgtggag ccagttctgt accctctgtt gcgcagagat ttggtggccc 12061 aaggcccacg ctatgtggtc cagattggag ataagatcat cgactataac gaggagtttc 12121 gcctgttcct gagcactagg aatcccaacc cattcattcc accagacgct gccagcatcg 12181 tcacagaggt taatttcaca accacccgct caggactgag aggccagctc ctggccctca 12241 ccatccaaca tgagaaaccc gatttggaag aacagaagac taagctgctc caacaggagg 12301 aggataagaa gatccaactg gccaaactcg aagaatctct gctggagaca ttggctactt 12361 ctcagggcaa catcctggag aacaaggacc tgatcgagtc tctgaatcag actaaagcat 12421 cttccgccct catccaagag tctctgaagg aatcttataa gctgcaaatc tctctcgacc 12481 aggagcgcga cgcatatctg ccactcgctg agtctgcttc aaagatgtac ttcattatct 12541 ctgacctctc caagatcaat aatatgtaca ggttctccct ggccgccttt ctgaggttgt 12601 ttcaaagggc actccagaat aagcaggatt ctgagaacac agaacagagg attcaatccc 12661 tgatcagctc cctgcagcac atggtgtacg aatacatctg ccgctgcctg ttcaaggccg 12721 accagctgat gttcgccctg catttcgtca gaggtatgca tccagaattg ttccaggaga 12781 acgaatggga taccttcact ggcgtggtgg tcggagacat gttgcgcaaa gccgactccc 12841 agcagaagat cagggaccag ttgccttcat ggatcgatca agagagatcc tgggctgtgg 12901 caaccttgaa gatcgctctg ccttccctgt accagactct gtgtttcgag gacgccgctt 12961 tgtggcgcac ctactacaac aacagcatgt gtgagcagga atttcctagc atcttggcta 13021 agaaggtcag cttgttccag cagatcctcg tcgtgcaggt gctcaggcca gatagattgc 13081 agtcagctat ggccctcttt gcctgcaaga ccttgggttt gaaggaagtc tctccactcc 13141 cactcaatct caagaggctg tacaaggaga cactcgaaat cgagcccatt ctgatcatta 13201 tttctccagg agccgatccc tcccaggaac tccaggagct cgccaacgcc gaaagatcag 13261 gagaatgtta ccaccaggtt gcaatgggcc agggccaagc tgacttggct atccaaatgc 13321 tcaaggaatg cgcaaggaat ggtgactggc tgtgtttgaa gaacttgcac ctggttgtct 13381 cctggctgcc tgtcttggag aaggaactga acaccttgca acccaaagac actttcaggc 13441 tctggttgac agccgaagtg cacccaaact ttacacctat cctcctccag tcaagcctca 13501 agatcactta tgaatcacca cctggactca agaagaatct catgcgcaca tatgaatctt 13561 ggacacctga acaaatctct aagaaagata acactcaccg cgcacatgct ctgttctctc 13621 tggcctggtt tcacgccgca tgccaagaga ggcgcaacta cattcctcaa ggttggacca 13681 aattctacga gttctctctc tccgacctga gggccggtta caatatcatt gacaggctct 13741 ttgatggtgc caaggacgtg caatgggagt ttgtccatgg tctgctggag aacgccatct 13801 acggaggccg catcgataac tatttcgact tgcgcgtcct gcagagctat ttgaagcaat 13861 tcttcaactc tagcgtcatt gacgtgttca atcagcgcaa caagaagtcc atctttccct 13921 acagcgtgag cctgccacaa agctgcagca ttctggatta cagggctgtc attgagaaga 13981 ttccagaaga cgataagcca tccttcttcg gtctgcctgc caacattgca cgctcatcac 14041 agcgcatgat ctcatctcag gtgatttccc aactccgcat cctgggccgc tctattactg 14101 caggctcaaa gttcgatcgc gagatctgga gcaatgaact cagcccagtg ctcaacctgt 14161 ggaagaaact gaaccagaac tccaacctga tccaccagaa ggtccctcct cccaatgacc 14221 gccaaggatc accaattctg tcctttatta tcttggagca gttcaacgcc atcagattgg 14281 tccagtcagt tcatcaatca ctggcagcct tgagcaaagt gatccgcggc actacactgc 14341 tctcatcaga agtccagaag ttggcctctg ccctgctcaa ccagaagtgc cctctggcct 14401 ggcaatccaa gtgggaggga cccgaagacc ctctgcaata tctcagaggc ctcgtggcta 14461 gagcactggc catccagaat tgggtcgata aagcagagaa gcaggccctc ttgtccgaaa 14521 cactggacct ctctgaattg ttccatcccg acacattcct gaacgccctc cgccaggaaa 14581 cagcaagggc tgttggaaga tcagtggatt ctctgaaatt tgtcgcctcc tggaagggta 14641 gactgcaaga ggccaaactc cagatcaaga tctcaggact gctcctggaa ggctgctcct 14701 tcgacggtaa tcaactgtcc gagaatcagc tcgacagccc aagcgtctct agcgttctgc 14761 catgcttcat gggttggatt cctcaagacg cttgcggccc atactcaccc gatgagtgca 14821 tttctttgcc agtgtacaca tccgctgagc gcgacagagt cgtgaccaac atcgatgtgc 14881 cttgcggtgg taatcaggat caatggattc agtgtggagc cgccttgttt ctcaagaatc 14941 aggtctaact cgaggcatgc ggtaccaagc ttgtcgagaa gtactagagg atcataatca 15001 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 15061 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 15121 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 15181 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactga 15241 tatcgcctag gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt 15301 ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc 15361 ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc 15421 cacagcgggg catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt 15481 gacaaaccgt catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc 15541 tcttcgttat taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc 15601 gaatgg //