LOCUS Exported 902 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Cas9 expression cassette carried by a yeast single-copy episome plasmid. ACCESSION . VERSION . KEYWORDS Cas9-NAT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 902) AUTHORS Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS TITLE Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. JOURNAL Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3. PUBMED 25281382 REFERENCE 2 (bases 1 to 902) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..902 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 65..81 /label=SK primer /note="common sequencing primer, one of multiple similar variants" primer_bind 65..81 /label=pBluescriptSK /note="For pBluescript vector" ORIGIN 1 tctattacaa ctttttttac ttcttgctca ttagaaagaa agcatagcaa tctaatctaa 61 gttttctaga actagtggat cccccgggaa aaatggacaa gaagtactcc attgggctcg 121 atatcggcac aaacagcgtc ggttgggccg tcattacgga cgagtacaag gtgccgagca 181 aaaaattcaa agttctgggc aataccgatc gccacagcat aaagaagaac ctcattggcg 241 ccctcctgtt cgactccggg gagacggccg aagccacgcg gctcaaaaga acagcacggc 301 gcagatatac ccgcagaaag aatcggatct gctacctgca ggagatcttt agtaatgaga 361 tggctaaggt ggatgactct ttcttccata ggctggagga gtcctttttg gtggaggagg 421 ataaaaagca cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg 481 aaaagtaccc aaccatatat catctgagga agaagcttgt agacagtact gataaggctg 541 acttgcggtt gatctatctc gcgctggcgc atatgatcaa atttcgggga cacttcctca 601 tcgaggggga cctgaaccca gacaacagcg atgtcgacaa actctttatc caactggttc 661 agacttacaa tcagcttttc gaagagaacc cgatcaacgc atccggagtt gacgccaaag 721 caatcctgag cgctaggctg tccaaatccc ggcggctcga aaacctcatc gcacagctcc 781 ctggggagaa gaagaacggc ctgtttggta atcttatcgc cctgtcactc gggctgaccc 841 ccaactttaa atctaacttc gacctggccg aagatgccaa gcttcaactg agcaaagaca 901 cc //