LOCUS Exported 911 bp ds-DNA linear SYN 19-MAY-2021 DEFINITION Cas9 expression cassette carried by a yeast single-copy episome plasmid. ACCESSION . VERSION . KEYWORDS Cas9-NAT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 911) AUTHORS Zhang GC, Kong II, Kim H, Liu JJ, Cate JH, Jin YS TITLE Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease. JOURNAL Appl Environ Microbiol. 2014 Dec;80(24):7694-701. doi: 10.1128/AEM.02310-14. Epub 2014 Oct 3. PUBMED 25281382 REFERENCE 2 (bases 1 to 911) AUTHORS . TITLE Direct Submission JOURNAL Exported May 19, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..911 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(22..40) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" misc_feature 81..584 /label=CEN/ARS /note="S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence" promoter 621..725 /gene="bla" /label=AmpR promoter ORIGIN 1 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 61 taataatggt ttcttagacg gatcgcttgc ctgtaactta cacgcgcctc gtatctttta 121 atgatggaat aatttgggaa tttactctgt gtttatttat ttttatgttt tgtatttgga 181 ttttagaaag taaataaaga aggtagaaga gttacggaat gaagaaaaaa aaataaacaa 241 aggtttaaaa aatttcaaca aaaagcgtac tttacatata tatttattag acaagaaaag 301 cagattaaat agatatacat tcgattaacg ataagtaaaa tgtaaaatca caggattttc 361 gtgtgtggtc ttctacacag acaagatgaa acaattcggc attaatacct gagagcagga 421 agagcaagat aaaaggtagt atttgttggc gatcccccta gagtctttta catcttcgga 481 aaacaaaaac tattttttct ttaatttctt tttttacttt ctatttttaa tttatatatt 541 tatattaaaa aatttaaatt ataattattt ttatagcacg tgatgaaaag gacccaggtg 601 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 721 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 781 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 841 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 901 gccccgaaga a //