LOCUS       Exported                 715 bp ds-DNA     linear   SYN 21-MAY-2021
DEFINITION  Expression vector for a sgRNA against the mouse Rosa26 locus and 
            Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A.
ACCESSION   .
VERSION     .
KEYWORDS    pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 gcaggtgacg tggaggagaa tcccgggcct cttaagatgg agagccgaaa tccttttcag
       61 cagggactgc cagccgggtt cctgtcttca tcctttgtgg agaatatgga ggtgccagcc
      121 cccgagtgca acctgcgact gctggcagga accgcagctc gccacagtga ggatcctgaa
      181 tcaccagcag ccggcggaag taggagagag agtgaatcac gaccaggacc ttcaggagga
      241 ggagtcgctg acctgttccc agaactgcat cgcgtgctga cacgaagctc ctctggccga
      301 gagaggggaa tcaagagaga gcggcacgat gaaaccaatc ataggattga gctgacagtg
      361 gggctgatgt ctcgcaaacg acctgagact gtctggtggt acgaagtgca gagcaccggc
      421 acagacgaag tgtccgtcat gcacgagagg ttttctctgg aacaggtgaa gacctgttgg
      481 ctggagcctg aagacgattg ggaggtggcc atcagaaact acgccaagct ggctctgaga
      541 ccagaccgga agtacaagat cactaagctg atcaacatcc ggaatgcttg ctatatcagc
      601 ggcaatggag cagaggtcga aatttgtctg caggataggg tggccttcag atgctgtatg
      661 atgaacatgt accccggggt ggtcgacatg gatggcgtga ctttcatgaa tatga
//
