LOCUS Exported 724 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter driven. ACCESSION . VERSION . KEYWORDS pL-CRISPR.EFS.GFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 724) AUTHORS Heckl D, Kowalczyk MS, Yudovich D, Belizaire R, Puram RV, McConkey ME, Thielke A, Aster JC, Regev A, Ebert BL TITLE Generation of mouse models of myeloid malignancy with combinatorial genetic lesions using CRISPR-Cas9 genome editing. JOURNAL Nat Biotechnol. 2014 Jun 22. doi: 10.1038/nbt.2951. PUBMED 24952903 REFERENCE 2 (bases 1 to 724) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..724 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 126..145 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 222..297 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 345..462 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 aggctgttag agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa 61 atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta 121 aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata 181 tcttgtggaa aggacgaaac accggagacg tgaccgtctc tgttttagag ctagaaatag 241 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt 301 tttgaattct agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg 361 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa 421 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac 481 agcagagatc cactttggcg ccggctcgag tggctccggt gcccgtcagt gggcagagcg 541 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta 601 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc 661 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa 721 cggg //