LOCUS       Exported                 800 bp ds-DNA     linear   SYN 20-MAY-2021
DEFINITION  shRNA against human pro-caspase 1.
ACCESSION   .
VERSION     .
KEYWORDS    shRNA Caspase 1 (pSicoR mCherry)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Doitsh G, Galloway NL, Geng X, Yang Z, Monroe KM, Zepeda O, Hunt PW,
            Hatano H, Sowinski S, Munoz-Arias I, Greene WC
  TITLE     Cell death by pyroptosis drives CD4 T-cell depletion in HIV-1 
            infection.
  JOURNAL   Nature. 2014 Jan 23;505(7484):509-14. doi: 10.1038/nature12940.
  PUBMED    24356306
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    287..320
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        520..731
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
       61 tccagtttgg ttagtaccgg gcccgctcta gagatccgac gcgccatctc taggcccgcg
      121 ccggccccct cgcacagact tgtgggagaa gctcggctac tcccctgccc cggttaattt
      181 gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac agataatctg
      241 ttctttttaa tactagctac attttacatg ataggcttgg atttctataa cttcgtatag
      301 catacattat acgaagttat acatgtcaca aaaggaaact caccctaact gtaaagtaat
      361 tgtgtgtttt gagactataa atatcccttg gagaaaagcc ttgtttacac gtcttgctct
      421 cattattcaa gagataatga gagcaagacg tgtttttttc tcgagtacta ggatccatta
      481 ggcggccgcg gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca
      541 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
      601 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
      661 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
      721 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta
      781 cctgaggccg ccatccacgc
//
