LOCUS       Exported                 604 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  Encodes RVD positions 4-6 in TALEN assembly.
ACCESSION   .
VERSION     .
KEYWORDS    pFusX2-12
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Ma AC, McNulty MS, Tanya PL, Jarryd CM, Martinez-Galvez G, Argue DP,
            Lee HB, Urban MD, Bullard CE, Blackburn PR, Man TK, Clark KJ, Ekker 
            SC
  TITLE     FusX: A rapid one-step TALE assembly system for genome science.
  JOURNAL   Hum Gene Ther. 2016 Feb 8.
  PUBMED    26854857
REFERENCE   2  (bases 1 to 604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(387..404)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(387..403)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(396..418)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      467..553
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cgtcacccgg caaccttggg tagcagcgaa gtcgaggcat ttctgtcctg gctggtctag
       61 acgtctcgcc atggtcctgg cacagcaccg gcaacagccg ctgcaccgtt tcgagcgctt
      121 gcttgccgcc accgttgctg gcgatagcca ccacttggtc cggggtcagg ccatggtcct
      181 ggcacagcac cggcaacagc cgctgcaccg tttcgagcgc ttgcttgccg ccattgttgc
      241 tggcgatagc caccacttgg tccggggtca ggccatggtc ctggcacagc accggcaaca
      301 gccgctgcac cgtttcgagc gcttgcttgc cgccaatgtt gctggcgata gccaccactt
      361 ggtccggggt cagggagacg cttaagactg gccgtcgttt tacaacgtcg tgactgggaa
      421 aacatccatg ctagcgttaa cgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
      481 ggctcagtcg gaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
      541 gagtaggaca aatccgccgg gagcggattt gaacgttgtg aagcaacggc ccggaagggt
      601 ggcg
//
