LOCUS Exported 645 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein. ACCESSION . VERSION . KEYWORDS pSB700 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 645) AUTHORS Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, Church GM TITLE Highly efficient Cas9-mediated transcriptional programming. JOURNAL Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312. PUBMED 25730490 REFERENCE 2 (bases 1 to 645) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..645 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 120..139 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 219..294 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" ORIGIN 1 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt 61 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg 121 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt 181 ggaaaggacg aaacaccgga gacgattaat gcgtctcggt tttagagcta gaaatagcaa 241 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt 301 tgaattctcg acctcgagac aaatggcagt attcgtcatt agttcatagc ccatatatgg 361 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 421 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 481 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 541 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 601 cccagtacat gaccttatgg gactttccta cttggcagta catct //