LOCUS Exported 4071 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Fluorescent reporter to monitor the activty of Bxb1. ACCESSION . VERSION . KEYWORDS Bxb1-surrogate-reporter SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4071) AUTHORS Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut P, Herzog F, Leonhardt H, Bultmann S TITLE A modular open platform for systematic functional studies under physiological conditions. JOURNAL Nucleic Acids Res. 2015 May 24. pii: gkv550. PUBMED 26007658 REFERENCE 2 (bases 1 to 4071) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4071 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 61..364 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 365..568 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 519..539 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" polyA_signal 859..980 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(896..915) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 950..969 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(987..1442) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1124..1145) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1336..1355 /label=F1ori-R /note="F1 origin, reverse primer" promoter 1469..1573 /gene="bla" /label=AmpR promoter promoter 1575..1932 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(1598..1618) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 1783..1918 /label=SV40 ori /note="SV40 origin of replication" primer_bind 1845..1864 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 1967..2761 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2021..2040) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 2631..2650 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(2949..2968) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 2993..3040 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 3369..3957 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3858..3877 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 661 gatcatgggt ttgtctggtc aaccaccgcg gtctcagtgg tgtacggtac aaacctaact 721 aactaagtac aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta 781 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg 841 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat 901 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 961 aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc 1021 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 1081 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 1141 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 1201 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 1261 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 1321 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 1381 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 1441 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat 1501 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 1561 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt 1621 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 1681 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 1741 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 1801 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 1861 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 1921 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 1981 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 2041 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 2101 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc 2161 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 2221 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 2281 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 2341 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 2401 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 2461 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt 2521 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 2581 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 2641 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 2701 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 2761 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat 2821 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc 2881 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg 2941 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata 3001 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc 3061 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt 3121 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac 3181 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta 3241 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 3301 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 3361 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 3421 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 3481 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 3541 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 3601 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 3661 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 3721 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 3781 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 3841 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 3901 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 3961 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 4021 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca t //