LOCUS       Exported                1137 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  harbours marker genes HIS3, LEU2 and URA3.
ACCESSION   .
VERSION     .
KEYWORDS    pHLU
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Michael Mulleder, Kate Campbell, Olga Matsarskaia, Florian 
            Eckerstorfer, Markus Ralser
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities
  JOURNAL   F1000Research
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(223..630)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 taacaaaacg gcatcagcct tcttggaggc ttccagcgcc tcatctggaa gtggaacacc
       61 tgtagcatcg atagcagcac caccaattaa atgattttcg aaatcgaact tgacattgga
      121 acgaacatca gaaatagctt taagaacctt aatggcttcg gctgtgattt cttgaccaac
      181 gtggtcacct ggcaaaacga cgatcttctt aggggcagac attagaatgg tatatccttg
      241 aaatatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa agtaagacga
      301 ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca acttcaagta
      361 ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag ccggttccgg
      421 cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg
      481 acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg atagcgcccc
      541 tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc gaactcttgc
      601 atcttacgat acctgagtat tcccacagtt aactgcggtc aagatatttc ttgaatcagg
      661 cgccttagac cgctcggcca aacaaccaat tacttgttga gaaatagagt ataattatcc
      721 tataaatata acgtttttga acacacatga acaaggaagt acaggacaat tgattttgaa
      781 gagaatgtgg attttgatgt aattgttggg attccatttt taataaggca ataatattag
      841 gtatgtagat atactagaag ttctcctcga tggcgcgcct gatgcggtat tttctcctta
      901 cgcatctgtg cggtatttca caccgcatag ggtaataact gatataatta aattgaagct
      961 ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta gttttgctgg
     1021 ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta
     1081 gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgta
//
