LOCUS Exported 10828 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentivirus-mediated expression of Jaws-ER2 under the CaMKII promoter.. ACCESSION . VERSION . KEYWORDS fck-Jaws-GFP-ER2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10828) AUTHORS Chuong AS, Miri ML, Busskamp V, Matthews GA, Acker LC, Sorensen AT, Young A, Klapoetke NC, Henninger MA, Kodandaramaiah SB, Ogawa M, Ramanlal SB, Bandler RC, Allen BD, Forest CR, Chow BY, Han X, Lin Y, Tye KM, Roska B, Cardin JA, Boyden ES TITLE Noninvasive optical inhibition with a red-shifted microbial rhodopsin. JOURNAL Nat Neurosci. 2014 Aug;17(8):1123-9. doi: 10.1038/nn.3752. Epub 2014 Jul 6. PUBMED 24997763 REFERENCE 2 (bases 1 to 10828) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10828 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2602..3890 /label=CaMKII promoter /note="promoter for the mouse calcium/calmodulin-dependent kinase II alpha subunit" CDS 4749..5462 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" CDS 4749..5462 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(4791..4812) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5052..5071) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 5399..5420 /label=EGFP-C /note="EGFP, forward primer" misc_feature 5520..6108 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(5573..5593) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5991..6002) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(6111..6127) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6112..6128) /label=pBluescriptKS /note="For pBluescript vector" LTR 6633..6813 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(6839..6856) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6845..7069 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 7115..7543 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7202..7221) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7412..7433 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7552..7572) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 7557..7886 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 7737..7872 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7799..7818 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 7934..7981 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 8000..8374 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 8504..8625 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(8541..8560) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 8595..8614 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(8674..8690) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8674..8690) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8687..8709) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8698..8714 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8722..8752) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8767..8788 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8905..8922) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9076..9664) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9156..9175) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9835..10695) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10458..10477 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10696..10800) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt 2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag 2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat 2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca 2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg 2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga 2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg 3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga 3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac 3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg 3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa 3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt 3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga 3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc 3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa 3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag 3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac 3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag 3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac 3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg 3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt 3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccg 3901 ccaccatgac cgccgtgagc accacagcca ctaccgtgct gcaggccaca cagagcgacg 3961 tgctgcagga gatccagtcc aacttcctgc tgaatagctc catctgggtg aacattgctc 4021 tggccggagt ggtcatcctg ctgtttgtgg ccatggggag ggatctggaa tcccctagag 4081 ctaagctgat ctgggtggcc acaatgctgg tgccactggt gtctatttct agttacgctg 4141 gactggccag tgggctgact gtgggcttcc tgcagatgcc acctggacac gctctggccg 4201 gacaggaggt gctgagccca tggggccggt atctgacatg gactttctcc actcccatga 4261 tcctgctggc tctgggactg ctggccgaca ccgatattgc cagcctgttc accgccatca 4321 caatggacat tggcatgtgc gtgacaggac tggccgctgc cctgatcact agctcccatc 4381 tgctgcgctg ggtgttctac ggaatttctt gtgctttctt tgtggccgtg ctgtatgtgc 4441 tgctggtgca gtggccagct gatgctgagg ctgctgggac cagtgaaatc tttggcactc 4501 tgaggattct gaccgtggtg ctgtggctgg ggtaccctat cctgttcgct ctgggctctg 4561 agggagtggc cctgctgagt gtgggagtga ccagctgggg atactccgga ctggacatcc 4621 tggctaaata cgtgttcgcc tttctgctgc tgagatgggt ggctgccaat gaaggcacag 4681 tgtctgggag tggaatggga atcgggtccg gaggagctgc tccagccgac gatcgaccgg 4741 tagtagcagt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc 4801 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca 4861 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc 4921 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca 4981 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca 5041 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca 5101 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg 5161 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga 5221 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc 5281 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca 5341 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca 5401 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca 5461 aggtagcatt ctgctacgag aatgaagtgt aataggaatt cgatatcaag cttatcgata 5521 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 5581 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 5641 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 5701 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 5761 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 5821 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 5881 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg 5941 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 6001 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc 6061 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc 6121 gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg 6181 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc 6241 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag 6301 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt 6361 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga 6421 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg 6481 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga 6541 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc 6601 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag 6661 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 6721 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 6781 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc 6841 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 6901 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 6961 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 7021 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg 7081 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc 7141 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 7201 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 7261 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 7321 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 7381 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 7441 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 7501 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt 7561 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 7621 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 7681 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 7741 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 7801 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 7861 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 7921 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga 7981 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg 8041 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt 8101 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca 8161 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct 8221 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat 8281 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa 8341 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac 8401 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 8461 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc 8521 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 8581 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc 8641 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg 8701 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 8761 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 8821 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 8881 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 8941 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 9001 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 9061 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 9121 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 9181 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 9241 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 9301 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 9361 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 9421 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 9481 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct 9541 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 9601 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 9661 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 9721 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 9781 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 9841 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 9901 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 9961 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 10021 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 10081 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 10141 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 10201 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 10261 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 10321 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 10381 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 10441 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 10501 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 10561 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 10621 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 10681 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 10741 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 10801 cacatttccc cgaaaagtgc cacctgac //