LOCUS       Exported                 998 bp ds-DNA     linear   SYN 02-JUN-2021
DEFINITION  harbours marker genes HIS3 and LEU2.
ACCESSION   .
VERSION     .
KEYWORDS    pHL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Michael Mulleder, Kate Campbell, Olga Matsarskaia, Florian 
            Eckerstorfer, Markus Ralser
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities
  JOURNAL   F1000Research
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     199..221
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     213..230
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     214..230
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     237..256
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        237..255
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    264..371
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(383..401)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(422..438)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(422..438)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(435..457)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    446..462
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(470..500)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    515..536
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(653..670)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(904..923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
       61 ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
      121 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
      181 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
      241 acgactcact atagggcgaa ttggagctcc accgcggtgg cggccgctct agaactagtg
      301 ggtcccccgg gctgcaggaa ttcgatatca agcttatcga taccgtcgac ctggaggggg
      361 ggcccggtac cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat
      421 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      481 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      541 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
      601 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
      661 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      721 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      781 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
      841 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      901 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      961 tgccgcttac cggatacctg tccgcctttc tcccttcg
//
