LOCUS       Exported                 831 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Expression of Cas9-6xHis in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    pET-Cas9-6xHis
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder
            ML, Joung JK, Chen ZY, Liu DR
  TITLE     Cationic lipid-mediated delivery of proteins enables efficient 
            protein-based genome editing in vitro and in vivo.
  JOURNAL   Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 
            2014 Oct 30.
  PUBMED    25357182
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    76..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             115..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat
       61 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      121 taactttaag aaggagatat accatggata agaaatactc aataggctta gatatcggca
      181 caaatagcgt cggatgggcg gtgatcactg atgaatataa ggttccgtct aaaaagttca
      241 aggttctggg aaatacagac cgccacagta tcaaaaaaaa tcttataggg gctcttttat
      301 ttgacagtgg agagacagcg gaagcgactc gtctcaaacg gacagctcgt agaaggtata
      361 cacgtcggaa gaatcgtatt tgttatctac aggagatttt ttcaaatgag atggcgaaag
      421 tagatgatag tttctttcat cgacttgaag agtctttttt ggtggaagaa gacaagaagc
      481 atgaacgtca tcctattttt ggaaatatag tagatgaagt tgcttatcat gagaaatatc
      541 caactatcta tcatctgcga aaaaaattgg tagattctac tgataaagcg gatttgcgct
      601 taatctattt ggccttagcg catatgatta agtttcgtgg tcattttttg attgagggag
      661 atttaaatcc tgataatagt gatgtggaca aactatttat ccagttggta caaacctaca
      721 atcaattatt tgaagaaaac cctattaacg caagtggagt agatgctaaa gcgattcttt
      781 ctgcacgatt gagtaaatca agacgattag aaaatctcat tgctcagctc c
//
