LOCUS       Exported                1025 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  harbours marker genes HIS3, LEU2, URA3 and LYS2.
ACCESSION   .
VERSION     .
KEYWORDS    pHLUK
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Michael Mulleder, Kate Campbell, Olga Matsarskaia, Florian 
            Eckerstorfer, Markus Ralser
  TITLE     Saccharomyces cerevisiae single-copy plasmids for auxotrophy 
            compensation, multiple marker selection, and for designing 
            metabolically cooperating communities
  JOURNAL   F1000Research
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        131..318
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     CDS             319..981
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
ORIGIN
        1 taattccgtt ttaagagctt ggtgagcgct aggagtcact gccaggtatc gtttgaacac
       61 ggcattagtc agggaagtca taacacagtc ctttcccgca attttctttt tctattactc
      121 ttggcctcct ctagtacact ctatattttt ttatgcctcg gtaatgattt tcattttttt
      181 ttttccacct agcggatgac tctttttttt tcttagcgat tggcattatc acataatgaa
      241 ttatacatta tataaagtaa tgtgatttct tcgaagaata tactaaaaaa tgagcaggca
      301 agataaacga aggcaaagat gacagagcag aaagccctag taaagcgtat tacaaatgaa
      361 accaagattc agattgcgat ctctttaaag ggtggtcccc tagcgataga gcactcgatc
      421 ttcccagaaa aagaggcaga agcagtagca gaacaggcca cacaatcgca agtgattaac
      481 gtccacacag gtatagggtt tctggaccat atgatacatg ctctggccaa gcattccggc
      541 tggtcgctaa tcgttgagtg cattggtgac ttacacatag acgaccatca caccactgaa
      601 gactgcggga ttgctctcgg tcaagctttt aaagaggccc taggggccgt gcgtggagta
      661 aaaaggtttg gatcaggatt tgcgcctttg gatgaggcac tttccagagc ggtggtagat
      721 ctttcgaaca ggccgtacgc agttgtcgaa cttggtttgc aaagggagaa agtaggagat
      781 ctctcttgcg agatgatccc gcattttctt gaaagctttg cagaggctag cagaattacc
      841 ctccacgttg attgtctgcg aggcaagaat gatcatcacc gtagtgagag tgcgttcaag
      901 gctcttgcgg ttgccataag agaagccacc tcgcccaatg gtaccaacga tgttccctcc
      961 accaaaggtg ttcttatgta gtgacaccga ttatttaaag ctgcagcata cgatatatat
     1021 acatg
//
