LOCUS Exported 819 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Expression of NLS-Cas9-6xHis in bacterial cells. ACCESSION . VERSION . KEYWORDS pET-NLS-Cas9-6xHis SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 819) AUTHORS Zuris JA, Thompson DB, Shu Y, Guilinger JP, Bessen JL, Hu JH, Maeder ML, Joung JK, Chen ZY, Liu DR TITLE Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo. JOURNAL Nat Biotechnol. 2015 Jan;33(1):73-80. doi: 10.1038/nbt.3081. Epub 2014 Oct 30. PUBMED 25357182 REFERENCE 2 (bases 1 to 819) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..819 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(3..47) /codon_start=1 /product="affinity and epitope tag derived from pancreatic ribonuclease A" /label=S-Tag /translation="KETAAAKFERQHMDS" CDS complement(66..83) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" ORIGIN 1 gacgagtcca tgtgctggcg ttcaaatttc gcagcagcgg tttctttacc agactcgagt 61 cattagtgat ggtggtggtg atggtcacct cctagctgac tcaaatcaat gcgtgtttca 121 taaagaccag tgatggattg atggataaga gtggcatcta aaacttcttt tgtagacgta 181 tatcgtttac gatcaattgt tgtatcaaaa tatttaaaag cagcgggagc tccaagattc 241 gtcaacgtaa ataaatgaat aatattttct gcttgttcac gtattggttt gtctctatgt 301 ttgttatatg cactaagaac tttatctaaa ttggcatctg ctaaaataac acgcttagaa 361 aattcactga tttgctcaat aatctcatct aaataatgct tatgctgctc aacaaacaat 421 tgtttttgtt cgttatcttc tggactaccc ttcaactttt cataatgact agctaaatat 481 aaaaaattca catatttgct tggcagagcc agctcatttc ctttttgtaa ttctccggca 541 ctagccagca tccgtttacg accgttttct aactcaaaaa gactatattt aggtagttta 601 atgattaagt cttttttaac ttccttatat cctttagctt ctaaaaagtc aatcggattt 661 ttttcaaagg aacttctttc cataattgtg atccctagta actctttaac ggattttaac 721 ttcttcgatt tccctttttc caccttagca accactagga ctgaataagc taccgttgga 781 ctatcaaaac caccatattt ttttggatcc cagtctttt //