LOCUS Exported 715 bp ds-DNA linear SYN 17-MAY-2021 DEFINITION Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain. ACCESSION . VERSION . KEYWORDS pHR-SFFV-dCas9-BFP-KRAB SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 715) AUTHORS Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman JS, Qi LS TITLE CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. JOURNAL Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. PUBMED 23849981 REFERENCE 2 (bases 1 to 715) AUTHORS . TITLE Direct Submission JOURNAL Exported May 17, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..715 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(35..55) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(62..82) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS complement(101..127) /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" ORIGIN 1 cgctgctgcc gttgctccca ctaccaatgc cgtccacttt ccgctttttc ttaggatctt 61 ccaccttgcg ttttttcttg ggggagccgc tgcccaggct ggcataatcg ggcacgtcat 121 agggataggc gtcgcctccc agctgagaca ggtcgatccg tgtctcgtac aggccggtga 181 tgctctggtg gatcagggtg gcgtccagca cctctttggt gctggtgtac ctcttccggt 241 cgatggtggt gtcaaagtac ttgaaggcgg caggggctcc cagattggtc agggtaaaca 301 ggtggatgat attctcggcc tgctctctga taggcttgtc tctgtgcttg ttgtaggcgc 361 tcagcacctt gtccagatta gcgtcggcca ggatcactct cttggagaac tcgctgatct 421 gctcgatgat ctcgtccagg tagtgtttgt gctgttccac aaacagctgt ttctgctcat 481 tatcctcggg ggagcccttc agcttctcat agtggctggc caggtacagg aagttcacat 541 atttggaggg cagggccagt tcgtttccct tctgcagttc gccggcagag gccagcattc 601 tcttccggcc gttttccagc tcgaacaggg agtacttagg cagcttgatg atcaggtcct 661 ttttcacttc tttgtagccc ttggcttcca gaaagtcgat gggattcttc tcgaa //