LOCUS       Exported                3546 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223-ABL2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Nov 24. ():.
  PUBMED    21107320
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             954..965
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atggggcagc aggtgggccg cgtcggggaa gctccggggc tccagcagcc tcagccccgc
       61 gggatccggg gcagcagtgc agccaggccc tccggccgca ggcgggaccc ggcggggcgc
      121 accacagaga ccggcttcaa tatcttcacc cagcatgatc actttgccag ctgtgtggag
      181 gatggatttg agggagacaa gactggaggc agtagtccag aagctttgca tcgtccctat
      241 ggttgtgatg ttgaacccca ggcactaaat gaggctatca ggtggagctc caaggagaac
      301 ttgctcggag ccactgagag tgaccctaat ctcttcgttg cactttatga ttttgtagca
      361 agtggtgata acacactcag catcactaaa ggtgaaaagc tacgagtcct tggttacaac
      421 cagaatggtg agtggagtga agttcgctct aagaatgggc agggctgggt gccaagcaac
      481 tacatcaccc cagtgaacag cctggaaaaa cactcctggt accatggacc tgtgtcacgc
      541 agtgcagctg agtatctgct cagcagtcta atcaatggca gcttcctggt gcgagaaagt
      601 gagagtagcc ctgggcagct gtccatctcg ctcaggtacg agggacgtgt gtatcactac
      661 aggatcaata ccactgcaga tggcaaggtg tatgtgactg ctgagagccg cttcagcacc
      721 ttggcagagc ttgtacacca tcactccaca gtggctgatg ggctggtgac aacattacac
      781 tacccagcac ccaagtgtaa taagcctaca gtctatggtg tgtcccccat ccacgacaaa
      841 tgggaaatgg agcgaacaga tattaccatg aagcacaaac ttgggggcgg tcagtatgga
      901 gaggtttacg ttggcgtctg gaagaaatac agccttacag ttgctgtgaa aacattgaag
      961 gaagatacca tggaggtaga agaattcctg aaagaagctg cagtaatgaa ggaaatcaag
     1021 catcctaatc tggtacaact tttaggtgtg tgtactttgg agccaccatt ttacattgtg
     1081 actgaataca tgccatacgg gaatttgctg gattacctcc gagaatgcaa ccgagaagag
     1141 gtgactgcag ttgtgctgct ctacatggcc actcagattt cttctgcaat ggagtactta
     1201 gagaagaaga atttcatcca tagagatctt gcagctcgta actgcctagt gggagaaaac
     1261 catgtggtaa aagtggctga ctttggctta agtagattga tgactggaga cacttatact
     1321 gctcatgctg gagccaaatt tcctattaag tggacagcac cagagagtct tgcctacaat
     1381 accttctcaa ttaaatctga cgtctgggct tttggggtat tgttgtggga aattgctacc
     1441 tatggaatgt caccatatcc aggtattgac ctgtctcagg tctatgacct actagaaaaa
     1501 ggatatcgaa tggaacagcc tgagggatgc ccccctaagg tttatgaact tatgagagca
     1561 tgctggaagt ggagccctgc cgataggccc tcttttgctg aaacacacca agcttttgaa
     1621 accatgttcc atgactccag catttctgaa gaggtagctg aggagcttgg gagagccgcc
     1681 tcctcgtcat ctgttgttcc atacctgccc cggctaccta tacttccttc caagactcgg
     1741 acactgaaga aacaggtgga gaacaaggag aacattgaag gggcacaaga tgccacagaa
     1801 aattctgctt ccagtttagc accagggttc atcagaggtg cacaggcctc tagtggatcc
     1861 ccagcactgc ctcgaaagca aagagacaag tcacccagca gcctcttgga agatgccaaa
     1921 gagacatgct tcaccaggga taggaagggg ggcttcttca gctccttcat gaagaagaga
     1981 aatgctccta caccccccaa acgcagcagc tccttccgag aaatggagaa tcagccccat
     2041 aagaaatacg aactcacggg taacttctca tctgttgctt ctctacagca tgctgatggg
     2101 ttctctttca ctcctgccca gcaagaggcg aatctggtgc cacccaagtg ctatgggggg
     2161 agctttgcac agaggaacct ctgtaatgac gacggtggtg ggggtggggg cagtggcact
     2221 gctgggggtg ggtggtctgg catcacaggc ttctttacac cacgcttaat caaaaagaca
     2281 ctgggcttac gagcaggtaa acccacagcc agtgatgaca cttccaagcc ttttccaagg
     2341 tcaaactcta catcttccat gtcctcaggg cttccagagc aggataggat ggcaatgacc
     2401 cttcccagga actgccagag gtccaaactc cagctggaaa ggacagtgtc cacctcttct
     2461 cagccagaag agaatgtgga cagggccaat gacatgcttc caaaaaaatc agaggaaagt
     2521 gctgctccaa gcagggagag accaaaagcc aagttattgc ccagaggagc cacagctctt
     2581 cctctcagaa caccctctgg ggatctagcc attacagaga aggaccctcc aggggtggga
     2641 gtggctggag tggcagctgc ccccaagggt aaagagaaga atggtggggc acgacttggg
     2701 atggctggag ttccagagga tggagagcag ccgggctggc cttctccagc caaggctgcc
     2761 cccgtcctcc caaccactca caaccacaaa gtgccagtcc ttatctcacc cactctgaaa
     2821 cacactccag ctgacgtgca gctcattggc acagactctc aggggaataa attcaagctc
     2881 ttatctgagc atcaggtcac atcctctgga gacaaggacc gaccccgacg ggtaaaacca
     2941 aagtgtgccc cacccccacc accagtgatg agactactgc agcatccgtc catctgctca
     3001 gaccctacag aagagccaac tgccctaact gcaggacagt ccacatcaga aacacaggaa
     3061 ggaggaaaga aggcagctct gggcgcagtg cccatcagtg ggaaagctgg gaggccagtg
     3121 atgcctccac ctcaagtgcc tctgcccaca tcttccatct cgccagccaa aatggccaat
     3181 ggcacagcag gtactaaagt ggctctgaga aaaaccaaac aggccgctga gaaaatctca
     3241 gcagacaaaa tcagcaaaga ggccctgctg gaatgtgctg acctactgtc cagtgcactc
     3301 acggaacctg tgcccaacag ccagctggta gacactggac accagctgct tgactactgc
     3361 tcaggctatg tggactgcat ccctcaaact cgcaacaaat ttgccttccg agaggctgtg
     3421 agcaaactgg aactcagcct gcaggagcta caggtttctt cagcagctgc tggtgtgccc
     3481 gggacaaacc ctgtccttaa taacttattg tcatgtgtac aggaaatcag tgatgtggtg
     3541 cagagg
//
