LOCUS Exported 981 bp ds-DNA linear SYN 30-MAY-2021 DEFINITION AAV packaging vector for hSyn driven KOR DREADD expression.. ACCESSION . VERSION . KEYWORDS pAAV-hSyn-dF-HA-KORD-IRES-mCitrine SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 981) AUTHORS Vardy E, Robinson JE, Li C, Olsen RH, DiBerto JF, Giguere PM, Sassano FM, Huang XP, Zhu H, Urban DJ, White KL, Rittiner JE, Crowley NA, Pleil KE, Mazzone CM, Mosier PD, Song J, Kash TL, Malanga CJ, Krashes MJ, Roth BL TITLE A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of Behavior. JOURNAL Neuron. 2015 Apr 29. pii: S0896-6273(15)00289-5. doi: 10.1016/j.neuron.2015.03.065. PUBMED 25937170 REFERENCE 2 (bases 1 to 981) AUTHORS . TITLE Direct Submission JOURNAL Exported May 30, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..981 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 434..467 /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." protein_bind 518..551 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." CDS complement(566..817) /codon_start=1 /product="C-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VC155 /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" primer_bind complement(608..629) /label=EGFP-C /note="EGFP, forward primer" primer_bind 957..976 /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" ORIGIN 1 acgaccgacc ccgacccact ggacaagcac ccaaccccca ttccccaaat tgcgcatccc 61 ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact gccagcttca 121 gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg cctcagcact 181 gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg ccaccttggt 241 cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga gatagggggg 301 cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg 361 ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcga gaaggtaccg gatcctctag 421 agtcgactcc ggaataactt cgtataggat actttatacg aagttatgca gaatggtagc 481 tggattgtag ctgctattag caatatgaaa cctcttaata acttcgtata gcatacatta 541 tacgaagtta tggcgcgccc tatcacttgt acagctcgtc catgccgaga gtgatcccgg 601 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca 661 gtttggactg gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg 721 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga 781 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc 841 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc 901 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct 961 tgtagttgcc gtcgtccttg a //